应用荧光定量PCR与常规方法检测解脲脲原体、沙眼衣原体的比较

Comparative Analysis of Ureaplasma Urealyticum and Chlamydia Trachomatis by Fluorescent Quantitative PCR Method and Conventional Method

应用荧光定量PCR(FQ-PCR)与常规方法(用培养法检测解脲脲原体,用胶体金法检测沙眼衣原体)检测147例疑似解脲脲原体(UU)感染标本,FQ-PCR法阳性率52.3%,敏感性100%,特异性71.4%;培养法阳性率33.3%,敏感性63.6%,特异性100%;149例疑似沙眼衣原体(CT)感染标本,FQ-PCR法阳性率8.1%,敏感性100%,特异性96.5%;胶体金法阳性率4.0%,敏感性50%,特异性100%。因此,FQ-PCR法具有更高的敏感性,但特异性低于常规方法。

Comparative analysis of ureaplasma urealyticum and chlamydia trachomatis by fluorescent quantitative PCR method and conventional method. The detection of ureaplasma urealyticum was used by culturing method and the detection of chlamydia trachomatis was used by colloidal gold-labeled technique. The author collected experimental samples from clinical patients. including 147 patients suspected UU infecting and 149 patients suspected CT infecting. The positive rate of UU detected by FQ-PCR and culturing method was 52.3% and 33.3% respeetively; The sensitivity and specificity by FQ-PCR is 100% and 71. 4%, respectively; The sensitivity and specificity by culture method are 63. 6% and 100%, respectively. The positive rate of CT detected by FQ-PCR and colloidal gold-labeled technique are 8. 1% and 4. 0% respectively; The sensitivity and specificity is 100% and 96.5 % by FQ-PCR respectively; The sensitivity and specificity is 50% and 100% by gold-labeled method, respectively. Compared with conventional methods, FQ-PCR has higher positive rates and sensitivity, but lower specificity.