山东省外观健康羊群中检出羊边界病病毒

Detection of Border Disease Virus(BDV) in Apparently Healthy Sheep in Shandong

2014年从山东省外观健康的牛羊采集鼻拭子样品共707份,提取其RNA,用流感病毒特异性引物进行RT-PCR检测。对RT-PCR扩增的阳性产物进行序列测定,意外发现其中1份羊拭子样品含有羊边界病病毒,并且该结果得到其他试验验证。结合我国2012年首次在安徽和江苏两地检出该病毒,以及当前我国羊群流通情况,推测该病毒可能在我国有所存在。该调研结果对分析各类RT-PCR检测的假阳性产生原因,有参考意义。并提示RT-PCR检测结果难以作为疫病或感染病诊断的确切依据。其诊断结果有时需要用荧光探针或测序进行进一步鉴定。

707 nasal swab samples were collected from apparently healthy cattle and sheep in Shandong province in 2014. RNA was extracted from these samples and detected by RT-PCR using the primers specific to influenza viruses. RT-PCR products of the positive samples were sequenced. Unexpectedly, border disease virus （BDV） was found in one sheep swab sample, which was further confirmed through two RT-PCR assays specific to BDV. It was assumed that BDV likely exist in China, for BDV had been detected in Anhui and Jiangsu provinces in 2012 and live sheep were in frequent movement. The result was also of reference for the analysis of false positive results in various RT-PCR assays. It was suggested that the RT-PCR results hardly provide exact evidences for disease or infection diagnosis. The diagnosis results should be further identified by fluorescence probe or sequencing sometimes.