摘要
为了确定猪流行性腹泻病毒的流行特点及变异规律,本研究对我国华东地区分离到的14株PEDV的ORF3基因进行了克隆和序列测定,并与国内外代表毒株的ORF3基因进行了比较分析。结果表明：我国华东地区检测的PEDV流行毒株的ORF3基因之间的同源性为92.4%～100%,与经典毒株CV777同源性为92.0%～96.4%,与PEDV变异株的同源性为95.1%～100%。根据ORF3基因的遗传进化分析,分离的14个PEDV毒株与CV777的遗传进化距离较远,与PEDV变异株的遗传进化距离较近,表明我国PEDV流行毒株以变异株为主,较以往的疫苗株CV777发生了较大的变异,因此需要开发新的疫苗,制定更具针对性的防控措施。
To determine epidemic characteristics and variation of porcine epidemic diarrhea virus（ PEDV）,the ORF3 genes of 14 PEDV strains isolated from East China were amplified with RT- PCR,cloned and sequenced. The results showed that 14 PEDV isolates share92. 4% ～ 100% amino acid identity with each other,92. 0% ～ 96. 4% with CV777,and 95. 1% ～ 100% with variant strains. The phylogenetic analysis based on ORF3 indicated that 14 PEDV isolates shared low homology with CV777,and shared high homology with variant strains. Our findings indicated that the pandemic strains of PEDV in East China were genetically quite distant from the previous strain CV777. New vaccines and measures are needed for the prevention and control of the variant strains widespread in China.
出处
《畜牧与兽医》
北大核心
2015年第3期34-38,共5页
Animal Husbandry & Veterinary Medicine
