旋毛虫生殖力的实验研究——成虫产幼虫影响因素的探讨

STUDIES ON THE FECUNDITY OF TRICHINELLA SPIPALIS:INFLUENCING FACTORS OF LARVIPOSITION BY ADULT TRICHINELLA SPIRALIS

实验动物不同对旋毛虫产幼虫数有影响,从大鼠小肠收集的成虫产虫数较长爪沙鼠和小鼠的明显为多;自雄性大鼠小肠所收集的成虫其产虫数较雌性大鼠的屈著为多;自大鼠小肠前半部所收集的成虫产虫数较小肠后半部者的著为多。感染大鼠的肌肉期幼虫用量对成虫的产虫数有影响,感染量越多,平均每条成虫产幼虫数越少。7日龄成虫其产虫数较5、9、11日龄的显著为多;在M—199或RPMT—1640完全培养液中成虫产虫数较在BME、MEM或Hank′s完全培养液中显著为多;37℃时培养的成虫其产虫数较25、30、35、40、42℃培养的显著为多。在20～160条/3ml培养液范围内成虫数不受培养密度的影响,在培养液中雌虫的产虫数不受雄虫存在与否的影响。

Several factors influencing the larviposition of adult trichinella spiralis in vitro and in vivo were studied. In the different experimental animal hosts, the fecundities of adult worms were different. In the rats, the jirds and mice, the adult worms recovered from the small intestine of the rats deposited the most larvae and those recovered from the small intestine of the mice shed the least larvae (P<0.01). Adult worms recovered from the small intestine of the female rats deposited fewer larvae than those isolated from the small intestine of the male rats (p<0.01).Adult worms isolated from the anterior half of the small intestine of the rats produced more larvae than those recovered from the posterior half of the small intestine of the rats (p<0. 01). As the level of infection in rats increased, the number of newborn larvae shed in vitro by adult worms decreased. The 7-day old adult worms (post-infection) deposited more larvae than did those of younger or elder than 7-day old worms (P<0.01). In the complete incubation solution of M-199 or RPMI-1640,the number of newborn larvae shed by adult worms was similar. and more than those shed by adult worms incubated in the complete incubation solution of BME,MEM or Hank's fluids (p<0.01). Adult worms incubated at temperature above and below 37℃ (42, 40, 35, 30, 25℃)shed fewer larvae than those incubated at 37℃(p<0.01). The mean number of newborn larvae shed in vitro by adult worms was independent of the number of female worms (20,40,80,160)present in equal volumes of incubation solution. Adult female worms deposited similar number of newborn larvae in virto in the presence and in the absence of adult male worms in the incubation solution.