甘肃省228株临床分离结核分枝杆菌DNA分型初步研究

Preliminary study on DNA-genotyping of clinically isolated strains of Mycobacterium tuberculosis in Gansu

目的了解目前甘肃结核分枝杆菌流行株的基因型特征,并评价两种基因分型方法的应用价值。方法收集甘肃省结核分枝杆菌临床分离菌株,分别采用多位点数目可变串联重复序列分析(multiple-locus variable number tandem repeat analysis,MLVA)和间隔区寡核苷酸分型(spacer oligonucleotide typing,Spoligotyping)技术进行基因分型,用BioNumerics软件进行聚类分析后,进行结果综合分析、比对。结果利用MLVA分型方法,228株结核分枝杆菌被分为4大基因群,7个主要基因型;Spoligotyping可将228株结核分枝杆菌分为4个基因群,8个主要基因型,其中最大的基因型为北京家族基因型。两种方法在基因型分型方面经卡方检验差异无统计学意义(χ2=0.06,P>0.05)。结论两种方法对结核分枝杆菌的分型效果良好,重复性高,操作简便,可试用于大规模分子流行病学调查。北京家族是甘肃最主要的流行基因群。

Objective To understand the genotyping features of epidemic strains of Mycobacterium tuberculosis in Gansu and to compare the two genotyping methods. Methods A total of 228 strains of Mycobacterium tuberculosis were isolated from patient samples and genotyped using MLVA and Spoligotyping methods.BioNumerics software was used for data analysis. Results Four clusters of gene and 7major genotypes were identified by MLVA method,meanwhile,8 major genotypes were determined by Spoligotyping method.Beijing family genotype was found to be a dominant one.There was no significant difference between the two genotyping methods（χ2=0.06,P〉0.05）. Conclusions The two genotyping methods are able to be used for molecular epidemiological survey because of their high efficacy,repeatability and easy to use.Beijing family genotype is the most common one in Gansu.