双抗体ELISA检测人血清弓形虫循环抗原的初步研究

PRELIMINARY STUDIES ON THE USE OF DOUBLE—ANTIBODY SANDWICH ELISA FOR DETECTION OF CIRCULATING ANTIGENS OF TOXOPLASMA IN HUMAN SERA

应用抗弓形虫抗体酶联免疫吸附试验夹心法(双抗体ELISA),检测不同人群血清循环抗原(CAg)。肉类加工人员、饮食服务人员的阳性率分别为9.3%(15/161)及3.9%(3/76),献血员未检出阳性者(0/72),肉类加工人员与献血员的CAg阳性率间差异非常显著(P<0.01),提示弓形虫感染与接触肉类食品似有密切关系。该法检测抗核抗体、类风湿因子、抗链球菌溶血素“O”等阳性者血清93份,均为阴性。方法的特异性试验的抑制率>70%;重现性试验的变异系数<10%。结果表明,该法具有较高的敏感性和特异性,认为可作为人体弓形虫急性感染的诊断方法。

This paper deals with the detection circulating antigens (CAg) in human sera by using double-PcAb sandwich ELISA. The positive rates of meat-packing workers and attendants of catering trade are 9.3%(15/161) and 3.9%(3/176) respectively,and that of blood donors is 0(0/72). The difference between meat-packing workers and blood donors is remarkably significant(p<0.01). It shows that the infection of toxoplasma appears to be in close contact with touching meat products. Furthermore, using this method to detect 93 sera which contain positive antinuclear antibody, rheumatoid factor and antistreptolysin'O', the reactions are all negative. As the results, the inhibition rate is more than 70% and the coefficient of variation is less than 10%. So this method is of comparatively high specificity and sensitivity, and can be used as a diagnostic method for acute toxoplasmosis.