胰腺癌中CIP2A、PI3K和Survivin的表达及临床意义

Expression and clinical significance of CIP2A, PI3K, and survivin in pancreatic carcinomas

目的 研究胰腺癌组织中蛋白磷酸酶2A癌性抑制因子（CIP2A）、磷脂酰肌醇3-激酶（PI3K）及Survivin的表达及其临床意义.方法 采用免疫组织化学法检测64例胰腺癌组织及癌旁组织中CIP2A、PI3K及Survivin的表达情况,并分析它们与临床病理参数的关系.结果 CIP2A在胰腺癌组织及癌旁组织中的阳性表达率分别为70.3％,5.6％;PI3K在胰腺癌组织及癌旁组织中的阳性表达率分别为73.4％,8.3％;Survivin在胰腺癌组织及癌旁组织中的阳性表达率分别为75.0％,2.8％,差异均有统计学意义（P＜0.05）.CIP2A、PI3K及Survivin在胰腺癌组织中的表达均与肿瘤分化程度、TNM分期、神经浸润及淋巴结转移有关（均P＜0.05）;经Spearman等级相关性分析,CIP2A与PI3K及Survivin在胰腺癌组织中的表达均呈正相关（均P＜0.05）.PI3K与Survivin在胰腺癌组织中的表达也呈正相关（P＜0.05）.结论 CIP2A参与了胰腺癌恶性进展,可能通过激活PI3K/蛋白激酶B（Akt）/Survivin信号通路促进胰腺癌发生、侵袭和转移.CIP2A有望成为治疗胰腺癌的一个靶向治疗基因.

Objective To investigate the expressions of cancerous inhibitor of protein phosphatase2A （CIP2A）,phosphatidylinositol 3-kinase（PI3K）,and survivin in pancreatic carcinomas and their clinical significance.Methods The expressions of CIP2A,PI3K,and survivin proteins were tested by immunohistochemistry in 64 cases of pancreatic carcinomas and adjacent paracancerous tissues.Results The positive rate of CIP2A in pancreatic carcinomas was significantly higher than adjacent paracancerous tissues （70.3% vs 5.6%,P 〈0.05）.Significant difference was observed in the expression rate of PI3K between the patients with pancreatic carcinomas and paracancerous tissues （73.4% vs 8.3%,P 〈0.05）.Significant difference was also observed in the expression rate of survivin between the patients with pancreatic carcinomas and paracancerous tissues （75.0% vs 2.8%,P 〈0.05）.CIP2A,PI3K,and survivin were significantly differentially expressed in pancreatic carcinoma among different tumor differentiation,tumor node metastasis （TNM） stage,and neural invasion and lymph node metastasis （all P 〈 0.05）.Spearman correlation analysis showed significantly positive correlation between the expressions of CIP2A and the others （PI3K and survivin） （both P 〈0.05）,and between the expressions of PI3K and surviving （P 〈0.05）.Conclusions CIP2A was involved in the development of pancreatic carcinomas and might activate the PI3K/Akt/survivin pathway.Our data identified CIP2A as a critical oncoprotein involved in cell proliferation,invasion,and metastasis.It could serve as a therapeutic target for pancreatic carcinomas.