榄香烯对H22肝癌荷瘤小鼠TRAF6、Caspase-8表达的影响

Effect of elemene on the expressions of TRAF6 and caspase-8 in H22 liver tumor tissues of mice

目的 观察榄香烯对H22肝癌荷瘤小鼠肿瘤组织中肿瘤坏死因子受体相关因子6（TRAF6）、半胱氨酸-天冬氨酸蛋白酶8（Caspase-8）基因表达的影响.方法 采用皮下接种H22肝癌细胞株的方法复制小鼠肝癌模型.将BALB/c小鼠40只按随机数字表法分4组（每组10只）：模型组,榄香烯低、高剂量组,顺铂组.处死小鼠后称取各组小鼠肿瘤的重量.采用荧光定量RT-PCR方法检测各组药物对小鼠肝癌肿瘤组织TRAF6、Caspase-8基因表达的影响.结果 榄香烯低、高剂量组的抑瘤率分别为24.2％、27.4％,顺铂（DDP）组抑瘤率为28.2％,与模型组比较,榄香烯低、高剂量组与DDP组小鼠肝癌瘤重明显减轻（P＜0.01）.与模型组比较,榄香烯低、高剂量组与DDP组肝癌小鼠肿瘤组织中的TRAF6 mRNA表达水平下降,而Caspase-8 mRNA表达水平升高,差异有统计学意义（P＜0.05）.结论 榄香烯具有抑制肝癌生长的作用,其分子机制可能是下调肿瘤组织TRAF6表达,上调Caspase-8表达,促进肝癌细胞凋亡达到抗肿瘤的作用.

Objective To investigate the effect of elemene on mRNA expressions of tumor necrosis factor （TNF） receptor associated factor 6 （TRAF6） and caspase-8 in tumor tissues of mice bearing hepatoma H22.Methods Forty BALB/c mice models bearing hepatoma H22 were established by subcutaneous inoculating tumor cells.Forty BALB/c mice were randomly divided into 4 groups：model group,low-and high-elemene dosage groups,and cisplatin group.The tumors after executing mice were weighted.The mRNA expressions of TRAF6 and caspase-8 in tumor tissues were detected by quantitative real-time reversetranscription polymerase chain reaction （RT-PCR）.Results The dosage of elemene could inhibit tumor growth.The inhibition ratio of cancer in the low-and high-elemene dosage and cisplatin group was 24.2%,27.4%,and 28.2%,respectively.It reduced significantly tumor weights（P 〈0.01）.Compared to the model group,the expression level of TRAF6 mRNA on tumors was decreased significantly,while the expression level of caspase-8 mRNA was increased significantly in the other groups（P 〈 0.05）.Conclusions The present results indicated that molecular mechanism of inhibition of liver cancer growth treated by elemene might be through down-regulating mRNA expressions of TRAF6 and caspase-8,promoting tumor cells apoptosis,and achieving the anti-tumor effect.