构建SLE患者novel microRNA差异性表达谱及其靶基因的功能分析

Spectrum construction of differently-expressed novel microRNA in SLE patients and function analysis of its target genes

目的构建系统性红斑狼疮(SLE)患者与健康对照者(NC)新小核糖核酸(novel microRNA)的差异性表达谱;对显著性差异表达的novel microRNA进行靶基因预测,对靶基因的GO(gene ontology)及KEGG(kyoto encyclopedia of genes and genomes)的功能进行分析,探讨SLE的发病机制。方法运用高通量测序技术(high-throughput sequencing)获得SLE与NC总的RNA,对总RNA进行长度分布、基因比对、RNA分类注释、RNA特有及公共序列统计后,运用Mireap软件预测novel microRNA。得到novel microRNA进行差异性表达分析,寻找两组之间具有显著性表达的novel microRNA。采用Targetscan软件对差异性表达的novel microRNA进行靶基因预测,并选取靶基因借用DAVID功能注释软件对其参与的生物学过程进行GO富集及KEGG通路分析。结果 61个novel microRNAs在SLE与NC组之间具有显著差异性表达,其中43个上调表达,18个下调表达。差异性表达novel microRNA的靶基因主要富集在细胞与小分子结合、细胞器官与细胞膜、细胞代谢中。靶基因KEGG通路主要体现在粘附复合体通路中。结论 SLE与NC的novel microRNA存在差异性表达。差异性表达novel microRNA的靶基因可能在SLE的发病机制与临床症状中起着重要作用,可能作为特异性靶点深入研究。

Objective To predict the novel microRNA in systemic lupus erythematosus （SLE） and healthy control （NC） groups by building the spectrum of the novel microRNA different expression. Then to analyze the functions of Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） of the target genes which come from differently expressed novel microRNA. Methods We obtained the total RNA by high- throughput sequencing technology; then the total RNA was subjected to length distribution, genome mapping, RNA annotation, common and specific sequences accounted. We used Mireap software to predict novel microRNA and made a comparison of novel microRNA between the two groups. Afterwards, we used Targetscan software to predict the target genes which came from differently expressed novel microRNA. By DAVID functional annotation software, we could identify the target genes participating in GO enrichment and KEGG pathway function. Results Sixty-one significantly differently expressed novel rnicroRNAs were found, including 43 up-regulated and 18 down-regulated ones. The target genes from differently expressed novel microRNA mainly participated in cellular binding, cellular organelle membrane, and cellular metabolic process of GO enrichment. For the KEGG pathways, the target genes mainly concentrated on the focal adhesion pathway. Gonclusion There is a different expression of novel microRNA between SLE and NC groups. The target genes from differently-expressed novel microRNA may play an important role in the pathogenesis of SLE and clinical symptoms and may be the unique target for further research.