摘要
目的探讨ALC1/CHD1L与OV6阳性肝癌细胞的关系及其对干性相关转录因子表达的影响。方法细胞免疫荧光标记方法观察肝癌Huh7及Hep G2细胞中ALC1/CHD1L和OV6的表达及定位;q RT-PCR检测ALC1/CHD1L sh RNA干扰后对肝癌Huh7及Hep G2细胞干性相关分子Oct4、Sox2、Nanog、Bmi1、β-catenin表达的影响。结果细胞免疫荧光标记结果显示肝癌Huh7细胞和Hep G2细胞中均有ALC1/CHD1L与OV6的表达,两者共表达率为34.3%及48.34%;ALC1/CHD1L sh RNA干扰及q RT-PCR实验结果显示敲减Huh7及Hep G2细胞ALC1/CHD1L表达可引起Oct4、Sox2、Nanog、Bmi1、β-catenin等干性相关分子表达下降,与对照组相比差异均有统计学意义(P<0.05)。结论肝癌Huh7及Hep G2 OV6阳性细胞中有ALC1/CHD1L表达;敲减ALC1/CHD1L表达可引起Huh7及Hep G2细胞干性相关分子Oct4、Sox2、Nanog、Bmi1、β-catenin表达明显下调。
Objective To explore the correlation between the ALC1 / CHD1 L and OV6 expression and the effects of ALC1 / CHD1 L on the expression of stemness related transcriptional molecules in liver cancer cells.Methods The immunofluorescence labeling method was applied to test the expression and localization of OV6 and ALC1 / CHD1 L in Huh7 and Hep G2 cells.The expression of stemness related genes Oct4,Sox2,Nanog,Bmi1 and β-catenin in Huh7 and Hep G2 cells were tested by realtime fluorescent quantitative PCR（q RT-PCR） after the cells treated with ALC1 / CHD1 L sh RNA.Results Immunofluorescent results showed that the ALC1 / CHD1 L and OV6 were co-localized in Huh7 and Hep G2 cells and its expression rate were 34.3% in Huh7 and 48.34% in Hep G2.The expression of stemness related moleculars such as Oct4,Sox2,Nanog,Bmi1,β-catenin were significantly decreased in Huh7 and Hep G2 cells after treating with ALC1 / CHD1 L sh RNA when compared with control groups（P〈0.05）.Conclusion ALC1 / CHD1 L co-exist with OV6 in Huh7 and Hep G2 cells and the m RNA level of ALC1 / CHD1 L is corelated with the expression of Oct4 、Sox2、Nanog 、Bmi1、β-catenin in Huh7 and Hep G2 cells.
出处
《解剖学研究》
CAS
2015年第1期1-5,共5页
Anatomy Research
基金
国家自然基金(30872508)
广州市教育局"羊城学者"科研项目(12A016G)
