维生素D_3对实验性肝损伤小鼠肝脏超微结构改变的影响

Influence of vitamin D_3 on ultramicrostructural changes of hepatic tissue in rats with experimental hepatic injury

目的:探讨维生素D3(vitamin D3,Vit D3)对四氯化碳(CCl4)诱小鼠实验性肝损伤肝组织超微结构的影响.方法:将25只SPF级♂Balb/c小鼠随机分为正常组、模型组、Vit D3低、中、高剂量干预组.干预组使用Vit D3预处理2 wk,Vit D3高、中、低剂量组分别每日给予Vit D3 15.0、7.5、l.0μg/k g腹腔注射,正常组与模型组腹腔注射等量生理盐水,Vit D3预处理1 wk后,模型组与各干预组每只小鼠隔日腹腔注射0.1%CCl4橄榄油溶液0.2 m L造模,正常对照组与模型组同步隔日腹腔注射生理盐水,CCl4给药1 wk后,全部小鼠眼眶采血和留取肝脏组织标本,光镜下观察肝组织HE染色的病理变化;常规方法测定血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate transaminase,AST);透射电镜观察肝脏超微结构的变化.结果:Vit D3高剂量组和中剂量组的肝脏指数和血清ALT、AST的水平则显著低于模型组(高剂量组:P=0.0000,P=0.0000,P=0.0002;中剂量组:P=0.0019,P=0.0005,P=0.0012),模型组小鼠的肝脏指数和血清ALT、AST的水平较正常组显著性升高(P=0.0000,P=0.0000,P=0.0000).各Vit D3干预组小鼠肝脏HE染色及超微结构的改善情况均明显优于模型组.结论:Vit D3对CCl4诱导急性肝损伤小鼠肝脏的超微结构具有一定的保护作用.

AIM： To investigate the effect of vitamin D3 （VitD3） on the ultramicrostructural changes of hepatic tissue in Balb/c mice with CC14 induced acute liver injury. METHODS： Twenty-five Balb/c mice were randomly and equally divided into five groups：a control group, a vehicle group, and high-, medium- and low-dose VitD3 groups. Mice in the three VitD3 groups were intraperitoneally injected daily with VitD315.0, 7.5, and 1.0 μg/kg, respectively. Mice in the control group and vehicle group were daily intraperitoneally injected with 0.9% NaCl. After 2 wk of treatment, the vehicle group and treatment groups were intraperitoneally injected with 0.1% CC14 in olive （0.2 mL/2 d） for 7 d. Meanwhile, the control group was given equal volume of 0.9% NaC1. At the end of administration of CC14 solution, eye blood and liver tissue samples were taken from all the mice. Alanine aminotransferase （ALT） and aspartate transaminase （AST） were detected routinely, and pathological changes in liver tissues were detected by HE staining. The ultramicrostructural changes of hepatic tissue were observed with an electron microscope. RESULTS： The levels of liver index, ALT, and AST were significantly lower in the high- and medium-dose VitD3 groups than in the vehicle group （high-dose group： P = 0.0000, 0.0000, 0.0002; medium-dose group： P = 0.0019, 0.0005, 0.0012）. Compared with the control group, the levels of liver index, ALT, and AST were significantly higher in the vehicle group （P = 0.0000 for all）. The improvement of histological changes in the treatment groups was significantly superior to that in the control group.