金银花DNA去甲基化酶基因的生物信息学分析

Bioinformatics analysis of DNA demethylase genes in Lonicera japonica Thunb

DNA去甲基化酶基因广泛存在于植物中,本文主要通过生物信息学的方法从金银花转录组中获得4个DNA去甲基化酶基因,分别为LJDME1、LJDME2、LJDME3和LJDME4,并预测其编码蛋白的理化性质和表达模式。系统进化树结果表明LJDME1、LJDME2聚为一类,LJDME3、LJDME4聚为一类,与拟南芥中的DME关系更为密切。基因表达分析表明,4个DNA去甲基化酶基因在变种间差异表达明显,LJDME1、LJDME2基因表达水平在红金银花中高于金银花,推测其可能参与调控金银花活性成分积累的过程,并且4个DNA去甲基化酶基因具有组织表达特异性。本研究为进一步解析金银花中DNA去甲基化酶的功能奠定了基础。

The DNA demethylase genes are widespread in plants. Four DNA demethylase genes （LJDME1, LJDME2, LJDME3 and LJDME4） were obtained from transcriptome dataset of Lonicera japonica Thunb by using bioinformatics methods and the proteins＇ physicochemical properties they encoded were predicted. The phylogenetic tree showed that the four DNA demethylase genes and Arabidopsis thaliana DME had a close relationship. The result of gene expression model showed that four DNA demethylase genes were different between species. The expression levels of LJDME1 and LJDME2 were even more higher in Lonicerajaponica var. chinensis than those in L. japonica. LJDME1 and LJDME2 maybe regulate the active compounds of L. japonica. This study aims to lay a foundation for further understanding of the function of DNA demethylase genes in L. japonica.