慢性乙型肝炎患者外周血Th17细胞及相关转录因子表达的检测

The detection of Th17 and transcription factors in patients with chronic hepatitis B

目的 了解慢性乙型肝炎（HBV）患者外周血Th17免疫应答情况及辅助性T淋巴细胞相互之间的关系.方法 采用胞内流式细胞术检测外周血Th17细胞分泌细胞因子IL-17的表达水平,同时用实时荧光定量PCR（Realtime PCR）检测58例慢性HBV患者及40例健康对照者中的IL-17、T-bet、GATA-3和FoxP3 mRNA表达水平.结果 慢性HBV患者外周血Th17细胞的百分比为4.72％±1.80％,明显高于健康对照3.56％±1.26％（P＜0.001）;IL-17 mRNA表达水平在HBV患者（4±0.49）比健康对照者（1.19±0.19）升高（P ＜0.0001）,T-bet mRNA表达水平在HBV患者（2.27±0.24）比健康对照者（1.10±0.13）升高（P＜0.05）,GATA-3 mRNA表达水平在HBV患者（2.29±0.16）比健康对照者（1.10±0.10）升高（P ＜0.0001）,FoxP3 mRNA表达水平在HBV患者（2.03±0.15）比健康对照者（1.05±0.10）升高（P ＜0.0001）.结论 Th17细胞参与了乙型肝炎病毒感染患者的免疫应答,并与Th1、Th2和Treg细胞之间存在一定的调节关系.

Objective To explore the immune response of T helper cells 17 in patients with hepatitis B virus （HBV） and the relation with Th1,Th2 and Treg cells.Methods The percentages of Th17 cells were detected by intracellular cytokine staining with flow cytometry.Using Realtime PCR method,we assayed the mRNA expression of IL-17,T-bet,GATA-3 and FoxP3 in 58 HBV patients and 40 healthy controls in our research.Results Compared with control group （3.56% ± 1.26%）,the percentage of Th17 cells in the peripheral blood of HBV group （4.72% ± 1.80%） was higher （P 〈0.001）.The mRNA of IL-17 expression of the HBV group （4 ±0.49） was higher than in healthy control group （1.19 ±0.19,P 〈 0.0001）.The mRNA of T-bet expression of the HBV group （2.27 ± 0.24） was higher than in healthy control group （1.10 ± 0.13,P 〈 0.05）.The mRNA of GATA-3 expression of the HBV group （2.29 ± 0.16） was higher than in healthy control group （1.10 ± 0.10,P 〈 0.0001）.The mRNA of FoxP3 expression of the HBV group （2.03 ±0.15） was higher than in healthy control group （（1.05 ±0.10,P 〈 0.0001）.Conclusions The Th17 cells participated in immune response of the hepatitis B virus infection patients,and there has certain adjustment relations among Th1,Th2 and Treg cells.