精氨酸酶Ⅰ在HIV感染者外周浅表淋巴结中表达的研究

Expression of arginase Ⅰ in peripheral superficial lymph node of HIV-infected individuals

目的 检测精氨酸酶Ⅰ （Arg 1）在HIV感染者外周浅表淋巴结中的表达并探究其与HIV/AIDS进展的关系.方法 根据HIV感染者外周血中CD4^＋T淋巴细胞计数将患者分组,应用免疫组化方法检测HIV感染者与非HIV感染者外周浅表淋巴结中精氨酸酶Ⅰ的表达,用SPSS17.0软件进行统计分析.结果 HIV感染者外周浅表淋巴结中精氨酸酶Ⅰ的表达明显高于非HIV感染者淋巴结（P＜0.01）.不同外周血CD4^＋T淋巴细胞计数的HIV感染者淋巴结中精氨酸酶Ⅰ的表达水平不同.CD4^＋T淋巴细胞计数≥350/ μl与200/μl≤CD4^＋T淋巴细胞计数＜350/μl的HIV感染者淋巴结中精氨酸酶Ⅰ表达水平差异无统计学意义（P＞0.05）,而外周血CD4^＋T淋巴细胞计数≥350/μl,200/ μl≥CD4 ^＋T淋巴细胞计数＜350/ μl的HIV感染者外周浅表淋巴结中精氨酸酶Ⅰ较CD4^＋T淋巴细胞计数＜ 200/μl的AIDS患者高,差异有统计学意义（P＜0.05）.且外周血CD4^＋T淋巴细胞与精氨酸酶Ⅰ的表达水平呈负相关性.结论 以上结果提示Arg Ⅰ在外周浅表淋巴结中的高表达,并与AIDS疾病的进展具有相关性.

Objective To investigate the expression of arginase Ⅰ （.Arg Ⅰ） in peripheral superficial lymph nodes of HIV-infected individuals and to explore their correlation with HIV/AIDS disease progression.Methods All the patients were divided to two groups according to the CD4 ^＋ T cell counts in peripheral blood,immunohistochemistry was used to detect expression of Arg Ⅰ in peripheral superficial lymph node of non HIV-infected and HIV-infected individuals.The data were statistically analyzed with SPSS17.0.Results Levels of Arg Ⅰ expression in peripheral superficial lymph node of HIV-infected individuals were higher than those of non HIV-infected lymph nodes （P 〈 0.01）.The expressions of Arg Ⅰ in patients with AIDS in different CD4 ^＋ T lymphocyte counts were distinct.Levels of Arg Ⅰ expression in peripheral superficial lymph nodes of CD4 ^＋ T lymphocyte counts ≥350/ μl and 200/ μl ≤ CD4 ^＋ T lymphocyte counts 〈 350/ μl AIDS patients had no significant difference （P 〉 0.05）,while there was significant difference between expression of Arg Ⅰ in CD4 ^＋ T lymphocyte counts ≥ 350/ μl,200/ μl ≤ CD4 ^＋ T lymphocyte counts 〈 350/ μl and CD4 ^＋ T lymphocyte counts 〈 200/ μl AIDS patients （P 〈 0.05）.In addition,statistical analysis of the above results showed that CD4 ^＋T cell counts in peripheral blood were negatively correlated with expression level of Arg Ⅰ.Conclusions Levels of Arg Ⅰ expression in peripheral superficial lymph node of AIDS patients were significantly high and associated with disease progression.