临夏回族17个Y染色体短串联重复序列基因座遗传多态性

Genetic polymorphism of 17 Y-STR loci in Hui population from Linxia

目的：调查研究17个Y染色体短串联重复序列（Y-STR）基因座及其单倍型在甘肃省临夏回族自治州回族人群中的分布情况.方法：应用AmpFlSTR（R） YfilerTM PCR荧光标记复合扩增系统,对424名回族无关男性个体血样进行17个y-STR位点的复合扩增,用ABI 3730xl遗传分析仪对扩增产物进行检测分析,数据分析使用GeneMapper ID v3.2.结果：DYS456,DYS389 Ⅰ,DYS389Ⅱ,DYS390,DYS458,DYS19,DYS393,DYS385a／b,DYS391,DYS439,DYS635,DYS392,YGATA H4,DYS437,DYS438和DYS448各位点遗传多样性（GD值）分布在0.471 3～0.968 0之间.17个Y-STR位点共同构成的单倍型361种.其单倍型多样性为0.999 5.结论：临夏回族17个Y-STR位点具有较高的遗传多态性,可为父权鉴定和父系进化研究提供有价值的遗传学资料.

Objective： To investigate the genetic polymorphisms of 17 Y chromosomal STRs （DYS19, DYS389 I , DYS389 Ⅱ, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438, DYS439, DYS437, DYS448, DYS456, DYS458, DYS635 and YGATAH4） of the Hui population in the Linxia Hui Autonomous Prefecture. Methods： The template DNAs were extracted from blood samples of 424 unrelated Hui population male individuals, and seventeen Y chromosomal STRs were amplified using the AmpF1STR YfilerTM PCR Amplification kit. Amphfied products were separated and detected using the ABI 3730xl Genetic Analyzer. The data were analyzed using GeneMapper ID v3.2. Results： The gene diversity ranged from 0.471 3 to 0.968 0 at DYS456, DYS389 I, DYS389 Ⅱ, DYS390, DYS458, DYS19, DYS393, DYS385a／b, DYS391, DYS439, DYS635, DYS392, YGATA H4, DYS437, DYS438 and DYS448. A total of 361 different haplotypes were observed. The haplotype diversity value calculated from all 17 loci combined was 0. 999 5. Conclusion： The 17 Y-STR loci in Hui population of Linxia Hui Autonomous Prefecture are highly affluent genetic polymorphic and can offer valuable genetic data for paternity testing and paternal genetic lineages evolution.