CdTe量子点DNA荧光纳米探针的合成及表征

Preparation and Characterization of CdTe QDs-DNA Fluorescent Nanoprobes

采用水相合成法合成了巯基乙酸(TGA)修饰的水溶性CdTe量子点,通过反相微乳液法制备了二氧化硅及壳聚糖修饰的核壳型复合荧光纳米粒子,将其与DNA吸附连接,得到CdTe量子点DNA荧光纳米探针。用扫描电镜、透射电镜、荧光光谱、红外光谱、紫外光谱、ζ电位等测试方法对产物的理化性质进行了分析表征。结果表明制备了表面富含氨基的复合荧光纳米粒子,其对DNA具有良好的吸附作用。

The water-soluble CdTe quantum dots were synthesized with Thioglycolicacid（TGA） as stabilizer in the aqueous solution, and the ammonified core /shell nanoparticles coated with TEOS and chitosan were synthesized with reverse microemulsion method. The nanocomposites were connected with DNA by electrostatic adsorption.The property and morphology of these particles were characterized by SEM, TEM, FL, IR, UV and Zeta Potential.The results showed that the amino-modified nanocomposites were synthesized successfully and had high adsorption capacity for DNA.