雌激素受体α-29位基因多态性与HBV相关原发性肝癌易感性的关系

Association of ERα-29 gene polymorphisms with susceptibility to HBV-related hepatocellular carcinoma

目的探讨甘肃地区人群雌激素受体（ER）α-29位多态性与HBV感染相关原发性肝癌（PHC）发生的关系,从基因水平上探讨PHC的发病机制。方法采用聚合酶链反应-限制性片段长度多态性法检测106例HBV感染相关PHC患者、98例健康对照人群的ERα-29位多态性。用基因计数法计算检验人群的等位基因频率,进行Hardy-Weinberg遗传平衡定律检验。基因型及等位基因频率比较采用χ^2检验。结果 HBV感染相关PHC患者ERα-29位多态性的TT基因型和T等位基因频率（31.1%,53.8%）明显高于对照组（11.2%,32.1%）,差异有统计学意义（χ^2值分别为3.449、3.840,P值均〈0.05）;CC基因型和C等位基因频率（23.6%,46.2%）明显低于后者（47.0%,67.9%）,差异有统计学意义（χ^2值分别为3.488、3.840,P值均〈0.05）;T等位基因发生HBV感染相关PHC的风险是C等位基因的2.46倍（OR=2.46,95%CI：1.64～3.69）。结论 ERα-29位T等位基因可增加HBV感染相关肝癌发病的风险。

Objective To evaluate the relationship between estrogen receptor - α - 29 （ ERα - 29） gene polymorphisms and the develop- ment of HBV - related hepatocellular carcinoma （HCC） in Gansu Province, China, and to investigate the pathogenesis of HCC at the gene level. Methods Gene polymorphisms of ERα - 29 were analyzed in 106 HBV - related HCC patients and 98 healthy individuals as normal controls using the polymerase chain reaction -restriction fragment length polymorphism technique. Population allele frequencies were calcu- lated using the gene counting method and then tested using the Hardy - Weinberg law of genetic equilibrium. Comparisons of genotype and allele frequencies between groups were performed using the 22 test. Results The frequencies of Tr genotype and T allele of ERα -29 gene in HBV - related HCC patients were significantly higher than those in the normal controls, i.e. , 31.1% and 53.8% vs. 11.2% and 32.1% （χ^2 = 3. 449, P 〈 0. 05 ; χ^2 = 3. 840, P 〈 0.05）. In contrast, the frequencies of CC genotype and C allele of ERα - 29 gene in HBV - related HCC patients were significantly lower than those in the normal controls, i.e. , 23.6% and 46.2% vs. 47.0% and 67.9% （χ^2= 3. 488, P 〈 0. 05 ; χ^2 = 3. 840, P 〈 0. 05 ）. Compared with those carrying C allele, carriers of T allele had an increased risk （2.46 - fold） of HBV - relat- ed HCC （ OR = 2. 46, 95% CI： 1.64 - 3.69）. Conclusion T allele of ERα- 29 gene can increase the risk of HBV - related HCC.