家族性圆柱瘤基因在病理性瘢痕中的表达

Expression of cylindromatosis in human pathological scars

目的:探讨家族性圆柱瘤基因(cylindromatosis,CYLD)在病理性瘢痕组织及成纤维细胞中的表达及意义。方法:收集临床手术切除的皮肤标本72例,分为正常皮肤组(20例)、正常瘢痕组(14例)、瘢痕疙瘩组(25例)、增生性瘢痕组(13例)。免疫组织化学技术(SP法)和Western blot检测各组织中CYLD蛋白的表达情况并对结果进行差异比较;另收集瘢痕疙瘩、增生性瘢痕及周围正常皮肤组织各10例,采用组织块贴壁法进行原代成纤维细胞培养,Western blot测定CYLD蛋白在3种成纤维细胞中的表达情况。结果:1免疫组化结果表明CYLD蛋白在A组(0.762±0.039)与B组(0.721±0.042)中的阳性表达明显高于C组(0.173±0.069)与D组(0.213±0.072),(F=213.832,P=0.000);C组、D组分别与A组比较(P值均为0.000),C组、D组分别与B组比较差异均具有统计学意义(P值均为0.000);2Western blot结果显示CYLD蛋白在正常皮肤(0.550±0.030)与正常瘢痕组织(0.442±0.016)中的表达明显高于瘢痕疙瘩(0.068±0.013)与增生性瘢痕(0.178±0.009)(F=424.685,P=0.000);3CYLD蛋白在正常成纤维细胞(0.564±0.037)中的表达同样明显高于瘢痕疙瘩(0.240±0.022)与增生性瘢痕成纤维细胞(0.256±0.038)(F=90.306,P=0.000)。结论:CYLD蛋白在病理性瘢痕成纤维中的表达低于正常皮肤与正常瘢痕,说明CYLD的低表达可能与病理性瘢痕的形成具有一定的关系。

Objective：To investigate the expression and significance of cylindromatosis（CYLD）in pathological scar tissues and fibroblasts. Methods：Seventy-two skin specimens were collected from clinical patients and were divided into normal skin group（20 samples）,normal scar group（14 samples）,Keloids group（25 samples）and hypertrophic scars group（13 samples）. Immunohistochemistry and Western blot were used to detect the protein expression of CYLD in each group and the results were compared. Keloids,hypertrophic scars and surrounding normal skin tissues（10 specimens of each group） were collected for primary fibroblasts culture with tissue adherent method. Expressions of CYLD protein in the three kinds of fibroblasts were detected by Western blot. Results：The results of immunohistochemistry showed that the positive expressions of CYLD protein in group A and group B were significantly higher than those in group C and group D（F=213.832,P=0.000）. There were statistically significant differences in expression of CYLD protein between group A and groups C,D（P=0.000）and between group B and groups C,D（P=0.000）. 2Results of Western blot showed that the expression of CYLD protein in normal skin tissues and normal scars tissues were higher than those of keloids and hypertrophic scars（F=424.685,P=0.000）. 3Expressions of CYLD protein in normal skin fibroblasts were also significantly higher than those of keloid fibroblasts and hypertrophic scars fibroblasts（F=90.306,P=0.000）. Conclusion：Expression of CYLD protein in pathological scar fibroblasts is lower than that of normal skin,indicating that the low expression of CYLD may play an important role in the formation of pathological scar.