CaSki细胞内HPV L1蛋白的表达

Expression of human papillomavirus late capsid protein 1 in CaSki cells

目的了解含人乳头瘤病毒(HPV)基因组的宫颈癌Ca Ski细胞中HPV晚期衣壳蛋白1(L1)的表达。方法以多价HPV L1小鼠单克隆抗体做探针,采用ELISA、免疫组化、Western blotting等方法,检测Ca Ski细胞内L1蛋白的表达;采用RT-PCR法检测细胞内L1 mRNA的表达。结果 Ca Ski细胞呈HPV L1阳性反应,显示细胞内有HPV L1蛋白存在;Ca Ski细胞内有L1 mRNA存在。结论包含HPV16混合基因组的Ca Ski细胞可以表达L1蛋白。

Objective To investigate the expression of human papil1omavirus（ HPV） late capsid protein 1（ L1） in CaSki cells. Methods Using polyvalent HPV L1 mice monoclonal antibody as the probe,the L1 protein expression in CaS ki cells was investigated by ELISA,immunohistochemistry and Western blotting. L1 mRNA expression was detected by RT-PCR. Results HPV L1 was strong positive in CaSki cells,indicating that L1 protein was expressed in CaSki cells. L1 mRNA was expressed in CaSki cells. Conclusion CaSki cells integrated HPV 16 DNA genome can express L1 protein.