摘要
目的探讨神经生长因子(NGF)转染的人脐带间充质干细胞(hUC-MSCs)减轻中枢神经系统功能损伤和促进中枢神经系统功能恢复的可行性。方法构建缺血、缺氧脑损伤的Wistar大鼠模型,同时分离培养hUC-MSCs,并将腺病毒介导的NGF转染hUC-MSCs。将转染成功后的hUC-MSCs移植入大鼠模型中,饲养4周后采用足印分析试验、平衡木试验分别对大鼠的运动功能进行评价。结果流式细胞仪检测提示hUC-MSCs分离培养成功,hUC-MSCs可过表达NGF蛋白。与hUC-MSCs组比较,NGF-hUC-MSCs组幼鼠右侧足印步态重复间距和平衡木通过时间均有统计学差异(均P<0.05),左侧足印步态重复问距无统计学差异(P>0.05)。结论NGF联合hUC-MSCs移植能促进实验大鼠的运动功能恢复,为中枢神经系统损伤修复和并发症预防提供了新的途径。
Objective To investigate the effect of human umbilical cord mesenchymal stem cel s （hUC- MSCs） trans-fected with nerve growth factor （NGF） on injury recovery of central nerves in rats. Methods The isolated and cultured hUC- MSCs were transfected with adenovirus- mediated NGF gene. Ischemia hypoxia model of central nervous system was in-duced in Wistar rats. The NGF- transfected hUC- MSCs were transplanted in the model rats. The motion function of rats was eval-uated with footprint test and balance beam test. Results The transfected hUC- MSCs successful y expressed NGF protein. The footprint test and balance beam test showed that NGF- transfected hUC- MSCs promoted motor function recovery in rats with is-chemic injury of central nerves. Conclusion NGF- transfected hUC- MSCs can improve damage repair of central nerve in rats.
出处
《浙江医学》
CAS
2015年第3期197-199,215,共4页
Zhejiang Medical Journal
基金
浙江省温岭市科技计划项目(2011WLCB0093)
关键词
人脐带间充质干细胞
神经生长因子
中枢神经损伤
Human umbilical cord mesenchymal stem cells
Nerve growth factor
Central nerve damage
