摘要
目的:研究川乌在加热前后生物碱变化。方法:采用LC-MS测定方法,Agilent Zorbax Extend-C18色谱柱(250 mm×4.6 mm,5μm);柱温为30℃;流动相为醋酸铵(氨水调pH 8)-乙腈(20 mmol·L-1);流速为1.0 mL·min-1;质谱采用正离子检测模式。结果:分析鉴定了川乌中13个生物碱类成分,其中9个生物碱在加热过程中的变化较明显。结论:本方法简便,具有较高的准确度和精密度,可用于生物碱变化的分析。
Objective： To analyze the changes of chemical constituents in Radix Aconiti Preparata before and after heating. Methods： The different extract of Radix Aconiti Preparata were detected by using HPLC/ESI-MS. Results： The constituents with significant differences before and after heating were identified. The results showed that a total of 13 chemical constituents were identified and nine constituents were significantly different before and after heating. Conclusion： The HPLC/ ESI-MS method is proved to have a high sensitivity and the result is accurate and reliable. The method could reflect the changes of multiple chemical constituents of Chinese materia medica in the course of processing.
出处
《中国现代中药》
CAS
2015年第3期208-211,共4页
Modern Chinese Medicine
基金
北京中医药大学自主选题项目(2013-JYBZZ-JS-039)
关键词
川乌
液质联用
成分变化
Radix Aconiti Preparata
HPLC/ESI-MS
chemical constituents
