摘要
对灰树花子实体95%乙醇提取物的不同萃取分部(石油醚分部、氯仿分部、乙酸乙酯分部、正丁醇分部和剩余分部)进行了化学成分的定性检验和体外抗肿瘤、抑制DPP-IV酶的活性研究。结果表明:灰树花子实体的醇提取物中可能含有生物碱、氨基酸或蛋白、有机酸、酚类或鞣质、甾体、蒽醌等物质。石油醚分部、氯仿分部、乙酸乙酯分部和正丁醇分部对正常细胞WPMY-1的增殖无抑制作用而对三种肿瘤细胞L1210、SW620、K562全部或部分的增殖有一定的抑制作用,其中氯仿分部对L1210细胞增殖的抑制作用最强,其IC50达到0.13μg/m L。这说明这四个分部具有抗肿瘤活性。剩余分部对肿瘤细胞L1210、K562和正常细胞WPMY-1的增殖均具有抑制作用,说明该分部可能具有细胞毒性。灰树花子实体的氯仿分部、乙酸乙酯分部和正丁醇分部具有较强的抑制DPP-IV酶的活性,这三个萃取分部对DPP-IV酶抑制的IC50分别为497.33、776.51、704.24μg/m L,该结果说明此三个分部具有降血糖的潜力。
Five fractions（ petroleum ether fraction,CHCl3 fraction,Et OAc fraction,n-Bu OH fraction and residual fraction） obtained from 95% ethanol extract of Grifola frondosa fruiting bodies were analyzed to identify the chemical components by the system preparative tests and screened for their antitumor and DPP-IV inhibitory activities. The results indicated that compounds such as alkaloids,amino acids or proteins,organic acids,phenols,tannins,steroids and anthraquinone existed in G. frondosa fruiting bodies. Petroleum ether fraction,CHCl3 fraction,Et OAc fraction and n-Bu OH fraction showed no inhibition to WPMY-1 normal cells but showed inhibition to the proliferations of L1210,SW620,K562 tumor cells in whole or part,especially CHCl3 fraction showed strong inhibition to the proliferation of L1210 tumor cell at IC50 of 0. 13 μg / m L. It indicated that these 4 fractions had antitumor activity. The residual fraction showed inhibition to L1210,K562 tumor cells and WPMY-1 normal cells,which demonstrated the residual fraction had cytotoxicity. Moreover,CHCl3 fraction,Et OAc fraction and n-Bu OH fraction showed strong inhibitory activity to DPP-IV at IC50 of 497. 33,776. 51 μg / m L and 704. 24 μg / m L respectively. These results indicated that these 3 fractions had probability of hypoglycemic activity.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第3期381-385,共5页
Natural Product Research and Development
基金
上海市科技兴农重点攻关项目(2012-2-9)
