响应面法优化紫山药花青苷提取工艺

Optimization of Extraction Process of Purple Yam Anthocyanins Using Response Surface Methodology

以紫山药为实验材料,以酸性乙醇为提取溶剂通过Box-Behnken响应面法及Design-Expert 8.0.6分析软件建立二次多项式数学模型优化紫山药花青苷的提取工艺及参数。研究表明,五种单因素对花青苷得率影响大小的顺序为X5(盐酸质量分数)>X2(时间)>X4(乙醇体积分数)>X3(料液比)>X1(温度)。并且温度和料液比、温度和乙醇体积分数、提取时间和料液比、提取时间和盐酸体积分数之间存在交互作用。得到紫山药花青苷最佳提取工艺参数为温度:81℃、时间:3.5 h、料液比:1∶25、乙醇体积分数:70%、盐酸质量分数:18‰。在此条件下花青苷平均得率达到4.928 mg/g,相对标准偏差为0.31%,与数学模型理论得率间的相对误差小于1.0%。

In this paper,purple yam was used as testing material. Anthocyanins from purple yam were extracted by acidic ethanol. The quadratic polynomial mathematical model of anthocyanins was set up and its extraction conditions were optimized by Box-behnken response surface method and Design-Expert 8. 0. 6 analysis software. On the basis of single-factor experiments,the relationships between main extraction conditions including extraction temperature（ X1）,extraction time（ X2）,ratio of solid to liquid（ X3）,ethanol concentration（ X4） and hydrochloric concentration（ X5） were investigated.Results showed that their effects were in the order of X5 X2 X4 X3 X1. The optimal extraction conditions were obtained as follows： extraction temperature was 81 ℃,extraction time was 3. 5 h,the ratio of solid to liquid was 1 ∶ 25,ethanol concentration was 70%,hydrochloric concentration was 18‰. Under these conditions,the average yield of anthocyanins was 4. 928 mg / g,the relative standard deviation was 0. 31%,and the relative error was less than 1. 0% compared with the mathematical model.