2013年崇左市流行性感冒实验室检测结果分析

Analysis on laboratory test results of influenza in Chongzuo City in 2013

目的对崇左市2013年流行性感冒(流感)实验室控检测结果进行分析,了解2013年崇左市流感病原学特征,为实验室诊断流感提供参考。方法采集2013年崇左市人民医院及各地送检的流感样病例咽拭子标本912份,应用实时反转录聚合酶链反应(real time RT-PCR)技术检测样本中的AM、BNS、H1HA、H3HA、SWH1 HA、H5HA、H9HA、H7HA、N9NA基因。结果 912份标本中,检测到乙型流感病毒核酸阳性19份,季节性流感病毒H1亚型核酸阳性0份,季节性流感病毒H3亚型阳性4份,甲型H1N1病毒特异性基因片段阳性58份,季性性H3和甲型H1N1流感病毒核酸同时阳性1份,均未检测到人感染高致病性禽流感中的H5HA、H9HA、H7HA、N9NA基因。甲型H1N1的检出率较高为6.36%(58/912),阳性构成比为70.73%。乙型流感病毒核酸阳性率为2.08%(19/912),季节性H1流感病毒阳性率为0.00%(0/912),季节性H3流感病毒阳性率为0.44%(4/912),季性性H3和甲型H1N1流感病毒核酸同时阳性率为0.11%(1/912)。均未检测出人感染H5,H9,H7N9禽流感病毒。结论甲型H1N1仍是2013年崇左市流感的主要毒株类型,流行趋势以散发为主,较2009年大暴发流行有所下降。

[Objective]To analyze the laboratory test results of influenza in Chongzuo City in 2013, understand the pathogenic characteristics, and provide a reference for laboratory diagnosis of influenza. [Methods]912 oropharyngeal swabs samples of influenza like illness cases were collected from the Chongzuo People＇s Hospital and other hospitals in 2013. The real-time RT-PCR was used to detect AM, BNS, H1HA, H3HA, SWH1 HA, H5HA, H9HA, H7HA and N9NA gene.[Results]Among 912 samples, 19 were positive for influenza B virus, no seasonal influenza subtype HI was detected, 4 were positive for seasonal influenza subtype H3, 58 were positive for specific gene fragment of influenza A （H1NI）, 1 sample was positive for both of seasonal influenza subtype H3 and influenza A （H1NI）. HSHA, H9HA, H7HA and N9NA gene of human infection with highly pathogenic avian influenza were not found. The detection of influenza A （H1NI） was 6.36%（58/912）, and the positive constitute was 70.73%. The positive rate of influenza B virus, seasonal influenza subtype HI and seasonal influenza subtype H3 was 2.08% （ 19/912 ）, 0.00% （0/912） and 0.44% （4/912）, respectively. The positive rate of seasonal influenza subtype H3 complicated with influenza A （H1NI） was 0.11% （1/912）. HS, H9 and H7N9 of human infection with influenza virus were not detected. [Conclusion]The influenza A （H1NI） is the dominant pathogen of influenza in Chongzuo City in 2013, and the epidemic trend is mainly sporadic, which decrease as compared the large outbreak in 2009.