摘要
目的研究天舒胶囊化学成分及大鼠ig天舒胶囊后血中移行成分。方法以UPLC-Q-TOF-MS作为分析方法,以阿魏酸、咖啡酸、盐酸川芎嗪、天麻素、5-羟甲基糠醛和藁本内酯作为对照,综合分析总离子流色谱图、提取离子流图及质谱图等信息,通过对比各色谱峰的保留时间及质谱图特征,确认天舒胶囊化学成分及大鼠ig天舒胶囊后血中移行成分。结果推断出天舒胶囊中24个化学成分,其中5个来源于天麻,其他19个来源于川芎;给药后血浆中共发现13个原型成分。结论为阐明天舒胶囊药效物质基础和完善质量标准提供依据。
Objective To study the chemical constituents of Tianshu Capsule in vivo and in vitro.Methods UPLC-Q-TOF-MS was used as the analytic method.The ferulic acid,caffeic acid,ligustrazine hydrochloride,gastrodin,5-hydroxymethy furfural,and ligustilide were used as the reference compounds.The information on the total ion chromatogram,extraction chromatogram and the mass spectrogram were synthetically analyzed to confirm the constituents of Tianshu Capsule in vivo and in vitro.Results Twenty-four compounds from Tianshu Capsule were detected among five constituents came from Tianma and 19 constituents came from Chuanxiong.After oral administration of Tianshu Capsule,13 compounds were absorbed into plasma.Conclusion The findings obtained from the study can provide the useful information for the determination of bioactive substances and the perfection of quality standard of Tianshu Capsule.
出处
《中草药》
CAS
CSCD
北大核心
2015年第5期649-653,共5页
Chinese Traditional and Herbal Drugs
基金
国家科技部"重大新药创制"项目:偏头痛治疗药物天舒胶囊大品种技术改造(2012ZX09201201-002)
