真菌诱导子对毛葡萄愈伤组织细胞次生代谢的影响

Effects of Fungal Elicitors on Secondary Metabolism in the Callus Cells of Vitis heyneana

以毛葡萄Vitis heyneana Roemer et Schultes愈伤组织为试验材料,研究了不同种类真菌水溶性诱导子及其不同浓度、不同添加时间和作用时间对毛葡萄愈伤组织细胞次生代谢产物的影响。结果表明:真菌诱导子的加入提高了毛葡萄细胞中总黄酮、总酚的含量及过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)的活性,在培养的第28d以80μg/mL浓度的诱导子处理毛葡萄愈伤组织效果最好。不同真菌来源的诱导子对毛葡萄愈伤组织次生代谢的影响效应不同。这些研究结果暗示了通过真菌诱导子调控葡萄次生代谢的可能性。

Effects of different fungal elicitors with different concentrations,application time and action time on some secondary metabolites in Vitis heyneana callus were studied.The results indicated that the fungal elicitors effectively increased the contents of flavonoids and total phenols,and the activities of peroxydase and phenylalnine ammonialyase in the Vitis heyneana callus cells.The most effective concentration of elicitors was 80g/mL,and the appropriate time of treatment was 28 days after culture of the callus.Elicitors from different species of fungi can cause different physiological effects on Vitis heyneana cells.These results implied the possibility to regulate the components of grape by means of fungal elicitors.