HPLC测定静脉给药山楂叶提取物大鼠血浆中三种黄酮含量

HPLC Determination for Three Flavonoids Contents in Rats Plasma after Intravenous Administration of Hawthorn Leaf Extract

目的:建立同时测定静脉给药山楂叶提取物后大鼠血浆中三种黄酮含量的HPLC方法。方法:采用HPLC,以黄芩苷为内标物,甲醇沉淀蛋白,Phenomsil C18(5μm,250×4.6 mm);流动相:甲醇-乙腈-四氢呋喃-0.4%醋酸水(6∶1.5∶18.5∶74);检测波长:332 nm。结果:实验表明VG药动参数AUC0→t与剂量的增长呈正比,VR、VIT在大鼠血浆中的AUC0→t与给药剂量不成正比,表明VR、VIT在大鼠血浆中遵循非线性药代动力学行为。结论:建立HPLC测定大鼠体内3种黄酮方法简单、专属性强,适用于黄酮类成分大鼠体内的药代动力学研究。

Objective： To establish the HPLC method for simultaneous determination of three kinds of flavonoids contents in rats plasma after intravenous administration of hawthorn leaf extract. Methods： The plasma samples were deproteinized with methanol after addition of internal standard（I. S.） baicalin. HPLC analysis was performed on a Phenomsil C18（5μm,250 mm × 4. 6mm） analytical column,using methanol-acetonitrile-tetrahydrofuran-0. 4% aqueous acetic acid（6： 1. 5： 18. 5： 74） as the mobile phase with UV detection at 332 nm. Results： The experiment results showed that pharmacokinetic parameters AUC0→tincreased with the VG doses. The AUC0→tof VR and VIT in rats plasma were not directly proportional to the administration dose.The results indicated that the pharmacokinetics of VR and VIT in rats obeyed nonlinear processes. Conclusion： The simple and specific HPLC method can be successfully applied during a pharmacokinetic study of flavonoids in rats plasma.