摘要
研究芦荟皮多糖的微波辅助提取工艺及其抗氧化能力。在单因素试验基础上,以微波时间、微波功率、料液比、水提温度为因素,采用Box-Benhnke试验设计,以多糖得率为响应值,进行响应面分析,并考察芦荟皮多糖的抗氧化活性。结果显示,芦荟皮多糖的最佳微波辅助提取条件为料液比1∶31(g/m L)、微波时间95 s、微波功率400 W、提取温度74℃,在此条件下芦荟皮多糖提取率为4.926%;所提芦荟皮多糖能够降低脂质过氧化物形成、清除DPPH自由基和羟自由基,具有较强的抗氧化能力,且在一定浓度范围内,其抗氧化能力与粗提物浓度呈现一定的剂量效应关系。
Response surface methodology（ RSM） was used to optimize the conditions for microwave-assisted extraction of polysaccharides from aloe peel,and the antioxidant activity of polysaccharides was also evaluated.Based on single-factor tests,Box-benhnken center composite experiment was carried out with four factors,including microwave time,microwave power,solid-liquid rate and extraction temperature.RSM was used to determine the efect of prime factors on the yield of polysaccharides. Besides,the antioxidaut activity of the polysaccharides was evaluated in vitro.The results showed that the optimal parameiers of microwave-assisted extraction were solid-liquid ratio 1∶31,microwave time 95 s,microwave power 400 W,extraction temperature 74 ℃.Under the optimal conditions,the polysaccharide extraction rate was 4. 926%. The antioxidant activity of the polysaccharides from aloe peels was found in a dose dependent manner.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2015年第1期149-156,共8页
Acta Agriculturae Universitatis Jiangxiensis
基金
江苏省农业科技自主创新基金产业技术体系类项目[CX(12)1005]
关键词
芦荟皮
多糖
微波辅助提取
响应面分析
抗氧化活性
aloe peels
polysachharides
microwave-assisted extraction
response surface methodology
antioxidant activity
