液相色谱仪检定用甲醇中胆固醇标准物质的研制

Preparation of Cholesterol in Methanol Reference Materials for Liquid Chromatography Verification

采用重量–容量法制备了液相色谱检定用200μg/m L的甲醇中胆固醇标准物质,分别用F检验和回归曲线法对研制的标准物质进行了均匀性和稳定性检验,对定值结果的不确定度进行了评定。结果表明,研制的甲醇中胆固醇标准物质具有良好的均匀性和稳定性,定值结果的相对扩展不确定度为2%(k=2)。该标准物质可用于液相色谱仪示差折光检测器、蒸发光散射检测器的检定。

200μg/mL cholesterol in methanol reference materials for liquid chromatography verification was developed by weight–volumetric method. Uniformity and stability of standard solution were examined byF test and regression curve method respectively. The results showed that the uniformity and stability of the reference materials were good. The uncertainty of concentration value was assessed. The relative expanded uncertainty of concentration value was 2%（k=2）. The reference materials can be used to test liquid chromatography refractive index detector and evaporative light scattering detector.