气相色谱法测定1-(5-溴-1H-吲唑-1-基)乙酮

Determination of 1-(5-Bromo-1H-Indazol-1-yl)Ethanone by Gas Chromatography

建立了测定1-（5-溴-1H-吲唑-1-基）乙酮纯度的气相色谱法。采用DB–5型色谱柱，程序升温，火焰光度检测器，外标法定量，1-（5-溴-1H-吲唑-1-基）乙酮在100～1000 mg／L范围内内线性关系良好，检出限为0.01 mg／L。测定结果的相对标准偏差为1.67%～1.89%（n=5），样品加标回收率为98.1%～107.9%。用该方法测定了自制的1-（5-溴-1H-吲唑-1-基）乙酮样品的纯度。该测定方法准确度高、专属性较强且灵敏度较高，适用于1-（5-溴-1H-吲唑-1-基）乙酮的质量控制。

The method was set up to determine the purity of 1-（5-bromo-1H-indazol-1-yl）ethanone by gas chromatography. DB–5 column was used with program temperatured, FPD as the detector and external standard method was used as the quantitative method. 1-（5-bromo-1H-indazole-1-yl）ethanone had good linear relationship in the concentration range of 100–1 000 mg/L, the detection limit was 0.01 mg/L. RSD were 1.67%–1.89%（n=5）, and the recoveries were 98.1%–107.9%. Self-made 1-（5-bromo-1H-indazol-1-yl）ethanone sample purity was determined by this method. The method is accurate, specific and high sensitive, it can be used for quality control of 1-（5-bromo-1H-indazol-1-yl）ethanone.