特发性矮小患儿血中p53及p21waf/cip1的表达

Expressions of p53 and p21 waf/cip1 in blood of children with idiopathic short stature

目的:观察特发性矮小(ISS)患儿血中p53及细胞周期调节基因p21waf/cip1的表达,探讨二者与ISS发病机制之间的关系。方法:采用实时荧光定量PCR及ELISA法检测30例ISS患儿(ISS组)和30名正常儿童(对照组)外周血中p53和p21waf/cip1 mRNA的表达水平及血清中P53和P21waf/cip1蛋白的含量。结果:ISS组患儿外周血中p53 mRNA的表达水平及血清P53的浓度与对照组相比,差异无统计学意义(t=1.044和0.701,P均>0.05),ISS组p21waf/cip1 mRNA的表达水平较对照组升高(t=4.606,P<0.001)。结论:p21waf/cip1可能通过非依赖p53途径参与ISS的发生。

Aim ： To investigate the expressions of p53 and p21waf/cipl in blood of children with idiopathic short stature（ISS） and to elucidate the pathogenesis of ISS. Methods： Real-time PCR was used to measure the expressions of p53 mRNA and p2lwaf/cipl mRNA in peripheral blood from 30 children with ISS（JSS group） and 30 normal control{control group） ; ELISA was used to measure the levels of P53 and P21waf/cipl in serum of the children in the 2 groups. Results： The expression of p53 mRNA and the concentration of serum P53 had no statistical significance between the 2 groups（ t = 1. 044 and 0. 701,P 〉 0.05）. The expression of p21waf/cipl mRNA in ISS group was significantly higher than those in the control group（ t = 4. 066 ,P 〈 0. 001 ）. Conclusion ： p21waf/cipl may be responsible for the pathogenesis of ISS, which might not depends on p53 pathway.