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左卡尼汀对玻璃化冷冻小鼠卵母细胞体外受精结局的影响 被引量:1

Effects of L-carnitine on in vitro-fertilized outcomes of mouse oocytes underwent vitrification
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摘要 目的:研究左卡尼汀对玻璃化冷冻小鼠卵母细胞的影响。方法:将卵母细胞分为新鲜组(存活卵数243个),玻璃化冷冻组(解冻卵数282个),左卡尼汀高、中、低剂量(2.0、1.0、0.5 g/L)组(解冻卵数分别为285、280和279个)。观察各组卵母细胞中GSH含量、ROS水平以及卵母细胞存活率、受精率、卵裂率、囊胚形成率。结果:5组卵母细胞GSH含量、ROS水平及卵母细胞存活率、受精率、卵裂率、囊胚形成率差异均有统计学意义(F=881.290、182.760及χ2=113.361、21.560、16.660、9.667,P均<0.05)。与新鲜卵母细胞比较,玻璃化冷冻卵母细胞中GSH含量降低,ROS水平升高,卵母细胞存活率、受精率、卵裂率均降低(P<0.05)。左卡尼汀呈剂量依赖性地增加玻璃化冷冻卵母细胞中GSH含量,降低ROS水平(P<0.05);高剂量左卡尼汀可明显改善玻璃化冷冻卵母细胞的存活率(P<0.05)。结论:左卡尼汀可通过抗氧化作用改善玻璃化冷冻卵母细胞的结局。 Aim:To study the effects of L-carnitine on the in vitro-fertilized outcomes of mouse oocytes underwent vit-rification.Methods:Oocytes were divided into fresh group (243 survival oocytes), vitrification group(282 thawed oo-cytes), L-carnitine high, medium and low dose (2.0, 1.0, 0.5 g/L) groups(thawed oocytes number was 285,280,279, respectively).The GSH content, ROS level in oocytes and survival rate , fertilization rate, cleavage rate, blastocyst forma-tion rate of oocytes in each group were observed .Results:There were significant differences in the GSH content ,ROS level in the oocytes and the survival rate , fertilization rate, cleavage rate, and blastocyst formation rate among the 5 groups(F=881.290,182.760, and χ2 =113.361,21.560,16.660,9.667,P〈0.05).Compared with those of the fresh group , the GSH content was lower ,ROS level was higher in the oocytes of vitrification group , and the survival rate , fertilization rate , and cleavage rate decreased(P〈0.05).L-carnitine could increase the GSH content , reduce ROS level in the oocytes un-derwent vitrification, and high dose L-carnitine could improve the survival rate (P〈0.05).Conclusion:L-carnitine may improve the outcomes of oocytes which undergo vitrification through antioxidant effects .
出处 《郑州大学学报(医学版)》 CAS 北大核心 2015年第2期277-280,共4页 Journal of Zhengzhou University(Medical Sciences)
关键词 玻璃化冷冻 卵母细胞 左卡尼汀 vitrification oocyte L-carnitine
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  • 1Chen C. Pregnancy after human ooeyte cryopreservation[ J ]. Lancet, 1986,1 ( 8486 ) : 884.
  • 2Lei T, Guo N, Liu JQ, et al. Vitrification of in vitro matured oocytes: effects on meiotic spindle configuration and mito- chondrial function[J]. Int J Clin Exp Pathol,2014,7(3) : 1159.
  • 3Rail WF, Fahy GM. Ice-free cryopreservation of mouse em- bryos at-196 degrees C by vitrification [ J ]. Nature, 1985, 313(63) :573.
  • 4Gupta MK, Uhm SJ,Lee HT. Effect of vitrification and be- ta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization[ Jl Fertil Steri1,2010,93 ( 8 ) :2602.
  • 5Wu GQ, Jia BY, Li JJ, et al. L-carnitine enhances oocyte maturation and development of parthenogenetic embryos in pigs [ J] Theriogenology ,2011,76( 5 ) :785.
  • 6Hashimoto S. Application of in vitro maturation to assisted reproductive technology[ J]. J Reprod Dev ,2009,55 ( 1 ) : 1.
  • 7黄映琴,覃爱平,孙惟佳,靳玉甫,李柳铭,欧奇志.抗氧化剂左卡尼汀在IVF中的应用[J].实用医学杂志,2013,29(2):299-301. 被引量:4
  • 8林春莲.人类卵母细胞玻璃化冷冻的研究进展[J].中国医学创新,2011,8(4):177-178. 被引量:3
  • 9Wang L, Fu X,Zeng Y, et al. Epinephrine promotes devel- opment potential of vitrified mouse oocytes [ J ]. Pak J Biol Sci,2014,17(2) :254.
  • 10Somfai T, Ozawa M, Noguchi J, et al. Developmental com- petence of in vitro-fertilized porcine oocytcs after in vitro maturation and solid surface vitrification:effect of cryopr- eservation on oocyte antioxidative system and cell cycle stage [ J ]. Cryobiology, 2007,55 ( 2 ) : 115.

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