左卡尼汀对玻璃化冷冻小鼠卵母细胞体外受精结局的影响

Effects of L-carnitine on in vitro-fertilized outcomes of mouse oocytes underwent vitrification

目的:研究左卡尼汀对玻璃化冷冻小鼠卵母细胞的影响。方法:将卵母细胞分为新鲜组(存活卵数243个),玻璃化冷冻组(解冻卵数282个),左卡尼汀高、中、低剂量(2.0、1.0、0.5 g/L)组(解冻卵数分别为285、280和279个)。观察各组卵母细胞中GSH含量、ROS水平以及卵母细胞存活率、受精率、卵裂率、囊胚形成率。结果:5组卵母细胞GSH含量、ROS水平及卵母细胞存活率、受精率、卵裂率、囊胚形成率差异均有统计学意义(F=881.290、182.760及χ2=113.361、21.560、16.660、9.667,P均<0.05)。与新鲜卵母细胞比较,玻璃化冷冻卵母细胞中GSH含量降低,ROS水平升高,卵母细胞存活率、受精率、卵裂率均降低(P<0.05)。左卡尼汀呈剂量依赖性地增加玻璃化冷冻卵母细胞中GSH含量,降低ROS水平(P<0.05);高剂量左卡尼汀可明显改善玻璃化冷冻卵母细胞的存活率(P<0.05)。结论:左卡尼汀可通过抗氧化作用改善玻璃化冷冻卵母细胞的结局。

Aim：To study the effects of L-carnitine on the in vitro-fertilized outcomes of mouse oocytes underwent vit-rification.Methods：Oocytes were divided into fresh group （243 survival oocytes）, vitrification group（282 thawed oo-cytes）, L-carnitine high, medium and low dose （2.0, 1.0, 0.5 g/L） groups（thawed oocytes number was 285,280,279, respectively）.The GSH content, ROS level in oocytes and survival rate , fertilization rate, cleavage rate, blastocyst forma-tion rate of oocytes in each group were observed .Results：There were significant differences in the GSH content ,ROS level in the oocytes and the survival rate , fertilization rate, cleavage rate, and blastocyst formation rate among the 5 groups（F=881.290,182.760, and χ2 =113.361,21.560,16.660,9.667,P〈0.05）.Compared with those of the fresh group , the GSH content was lower ,ROS level was higher in the oocytes of vitrification group , and the survival rate , fertilization rate , and cleavage rate decreased（P〈0.05）.L-carnitine could increase the GSH content , reduce ROS level in the oocytes un-derwent vitrification, and high dose L-carnitine could improve the survival rate （P〈0.05）.Conclusion：L-carnitine may improve the outcomes of oocytes which undergo vitrification through antioxidant effects .