湘酿1号刺葡萄离体快繁技术试验

Rapid propagation technology of Xiangniang-1(Vitis davidii Foёx) in vitro

以刺葡萄品种湘酿1号的单芽茎段为材料,通过采用不同消毒方法和生根处理,以建立高效的离体快繁体系。结果表明:单芽茎段用1 mol/L HCl处理30 s后,再用0.1%升汞消毒8 min,接种在加入了20 mg/L四环素培养基中,能取得最优消毒效果;最优外植体诱导培养基为MS+6-BA 1.00 mg/L+IBA0.25 mg/L。与其他葡萄品种相比,"湘酿1号"生根能力弱。

The single bud green cuttings from Xiangniang-1（Vitis davidii Foёx） was used as materials to establish the rapid propagation system in vitro. The results showed that the green cuttings with single bud could obtain the best disinfection by 1 mol/L HCl for 30 s, and 0.1% HgCl2 for 8 min, finally them were inoculated in solid medium with 20 mg/L tetracycline. The optimum inducing medium on these explants was MS＋6-BA 1.0 mg/L＋IBA 0.25 mg/L. Compared with the other varieties, Xiangniang-1＆#39;s rooting ability was weak.