微生物絮凝剂生产菌T1的鉴定及其对生活污水絮凝特性

Identification of flocculant-producing strain t1 and its flocculation characteristics in domestic sewage

从活性污泥中分离筛选絮凝剂产生菌,得到一株对高岭土悬浊液具有较高絮凝活性的微生物絮凝菌T1.根据菌株的生理生化鉴定以及16S r DNA基因序列分析,确定该菌株为芽孢杆菌(Bacillus sp),该菌的絮凝活性物质分布在发酵液上清液中.通过正交实验确定最佳生活污水絮凝条件为絮凝剂投加量5%,污水p H值7.0,助凝剂Ca Cl2投加量为2%,在此条件下,T1发酵上清液对生活污水的絮凝率为83.85%.

An efficient flocculant-producing strain T1 was isolated from active sludge, which had a good flocculation effect on kaolin clay suspension. Based on morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis, strain T1 was classified as Bacillus sp. And the flocculants were mostly concentrated in the supernatant. The suitable flocculating conditions determined by orthogonal test are bioflocculants dosage 5%, the pH of wastewater value 7.0 and the coagulant CaC12 2%. The flocculation rate of T1 fermentation supernatant against domestic sewage was 83.58% under these conditions.