天香丹对动脉粥样硬化秽浊痰阻证ApoE-/-小鼠的实验研究

Experimental Study of Tianxiang Dan on ApoE-/- mice with Atherosclerosis Huizhuo Tanzu Syndrome

目的观察天香丹对动脉粥样硬化(AS)秽浊痰阻证Apo E-/-小鼠血脂水平、主动脉内皮细胞的影响,探讨其抗AS秽浊痰阻证的机制。方法将12只Apo E-/-小鼠正常对照组以普通饲料喂养,48只Apo E-/-小鼠以人工气候箱模拟新疆寒燥环境,高脂饲料喂养12周建立AS秽浊痰阻证模型。造模成功后随机分为天香丹组、阿托伐他汀组、荷丹片组、模型组,灌胃12周后处死,摘眼球取血测定血脂含量,取主动脉电镜观察病理学改变。结果与正常对照组比较,模型组小鼠血清TC、LDL-C含量上升,与模型组比较,天香丹组小鼠的TC、LDL-C含量下降,TG、HDL-C含量无显著差异,透射电镜观察显示天香丹组主动脉的内皮细胞病变程度明显轻于模型组。结论天香丹可有效降低AS秽浊痰阻证Apo E-/-小鼠TC、LDL-C含量,对动脉粥样硬化形成有抑制作用。

Objective： To observe the effect and the meachanism of Tianxiang Dan on atherosclerosis of Huizhuo Tanzu syndrome of Apo E-/- mice serum lipid levels and aortic endothelial cells.Methods： 12 Apo E-/- mice in the normal control group,normal diet,48 Apo E-/- mice by feeding with high fat diet and the use of artificial climate box to simulate Xinjiang dry and cold environment and fed for 12 weeks to establish As Huizhuo Tanzhu syndrome model,the rats were randomly divided into Tianxiang Dan group,atorvastatin statins group,traditional Chinese medicine control group,model group.Intragastric administration for 12 weeks.The eyeball blood was collected to determine the content of lipid,the pathological changes were observed in aorta electron microscope.Results： Compared with the normal control group,model group mice increased serum TC and LDL levels.Compared with the model group,TC and LDL-C decreased in Tianxiang Dan group mice,TG,HDL content had no significant effect.TEM showed Tianxiang Dan group aortic endothelial cell lesion were significantly lighter than that of model group.Conclusion： Tianxiang Dan,effectively reducing AS Huizhuo Tanzhu syndrome mice of Apo E-/-TC and LDL-C,can inhibit the formation of atherosclerosis.