益气养阴方对大鼠肺纤维化的干预作用及对Smad2、Smad7蛋白的影响

Effect of Yiqiyangyin Decoction on pulmonary fibrosis rat and expression of Smad2/Smad7 protein

目的:观察益气养阴方对博来霉素所致大鼠肺纤维化的干预作用及对Smad2、Smad7表达水平的影响。方法:选用雄性SD大鼠60只,随机分为正常组、模型组、中药组、激素组、中药+激素组5组。正常组大鼠经气管注入生理盐水,其余组大鼠气管内缓慢注入博来霉素5 mg/kg。自造模后第2天开始给药,中药组给予益气养阴方1 ml/100 g灌胃;激素组给予35%醋酸泼尼松溶液按1 ml/100 g体重灌胃;中药+激素组给予中药加激素混合溶液按1 ml/100 g灌胃;正常组和模型组予等量生理盐水灌胃。实验第28天处死所有大鼠,取左肺下叶行HE染色观察肺组织病理学改变及免疫组化检测大鼠肺组织Smad2、Smad7蛋白的表达。结果:与正常组相比,模型组大鼠肺泡炎和肺纤维化程度评分明显增高;激素组、中药组、中药+激素组大鼠肺泡炎及肺纤维化程度与模型组相比均减轻,以中药组肺损伤减轻更为明显。模型组肺泡上皮细胞及成纤维细胞胞浆中Smad2蛋白高表达,且表达量明显高于正常组;与模型组相比,激素组、中药组、中药+激素组Smad2阳性表达均减少,以中药组表达量最低。模型组Smad7蛋白较正常组明显减少;与模型组相比,激素组、中药组、中药+激素组Smad7蛋白表达增强,以中药组表达量最高。结论:益气养阴方对博来霉素所致大鼠肺纤维化具有干预治疗作用,其机制可能与抑制炎症反应、减少胶原蛋白的形成和沉积及干预Smad信号通路有关。

Objective：To observe the effect of Yiqiyangyin Decoction （YQYY） on bleomycin-induced rats pulmonary fibrosis and the expression of Smad2 and Smad7. Methods： Sixty male SD rats were randomly divided into normal group, model group, YQYY group, hormone group, YQYY plus hormone group. The rats in the normal group injected saline via the trachea, the rest rats injected ble- omyein（5 mg/kg）. From the second day of injection, YQYY group were treated with YQYY decoction（ 1 ml/lO0 g） ;hormone group were given 35% Prednisone acetate solution（ 1 ml/lO0 g） ;YQYY plus hormone group were treated with the mixture of YQYY decoction and hormone （1 ml/100 g） ;normal group and model group were given equivalent saline. After twenty-eight days, all the rats were killed, the rat lung histopathology was examined with HE staining and detect Smad2/Smad7 protein with immunohistochemical staining. Results： Compared with normal group, the model group rats alveolitis and pulmonary fibrosis scores were significantly increased;compared with model group, the hormone group, YQYY group, YQYY plus hormone group rats alveolitis and lung fibrosis scores were reduced, and the lung injury of YQYY group reduced obviously. The expression of Smad2 protein of model group in alveolar epithelial cells and the fibroblasts was significantly higher than normal group;compared with model group, the positive expression of Smad2 in hormone group, YOYY group, YQYY plus hormone group were reduced, the Smad2 of YOYY group was the lowest. Compared with normal group, the smad7 protein of model group decreased significantly;compared with model group, Smad7 protein expression in hormone group, YQYY group, YQYY plus hormone group were increased, the Smad7 expression of YQYY group was highest. Conchmion： Yiqiyangyin decoction has a good intervention and therapeutic effect on bleomycin-induced rats pulmonary fibrosis, its mechanism may be related to inhibiting the inflammatory reaction, reduce the formation and deposition of collagen and the intervention of Smad signal pathway.