紫椴种皮透性及种子浸提物生物效应的研究

Water Permeability of Seed Coat and Bio-effect of Seed Extraction Material of Tilia amurensis

采用称重和生物测定的方法,对紫椴种皮透水性及种子各部分浸提物的生物效应研究,实验结果表明,紫椴种子种皮透水性较差,不易吸水。紫椴种子的胚乳、种皮、果皮中确实都含有萌发抑制类的物质,按照其抑制强度从大到小依次为种皮、果皮、胚乳(胚),且该抑制物质浸提液浓度高则抑制性强,浸提液浓度低则抑制性弱。紫椴种子胚各有机相及水相对白菜种子发芽率的抑制活性从大到小依次为:乙酸乙酯相,石油醚相,乙醚相,水相,甲醇相;对白菜种子胚根长抑制活性从大到小依次为:石油醚相,水相,乙酸乙酯相,乙醚相,甲醇相。种皮的各有机相及水相对白菜种子发芽率和胚根长的抑制活性从大到小依次为:甲醇相,水相,乙醚相,乙酸乙酯相,石油醚相。果皮的各有机相及水相对白菜种子发芽率和胚根长的抑制活性从大到小依次为:甲醇相,乙酸乙酯相,石油醚相,乙醚相,水相。

The testa permeability of Amur linden seed and biological effect of seed extract were studied using the weight and biological assay methods. Results showed that the testa had poor permeability for the waterproof compact layer. There was germination inhibitors in the pericarp,testa and endosperm（ embryo） and the inhibitory activities was ordered as testa pericarp endosperm（ embryo）,which had a positive correlation with the concentration. The inhibitory activities for the percentage germination of B. pekinensis seedlings of all organic phases and water phase in the embryo was ordered as follows： ethyl acetate phase,petroleum ether phase,ethylether phase,water phase,methanol phase; while the order for the root growth of B. pekinensis seedlings was petroleum ether phase,water phase,ethyl acetate phase,ethylether phase,and methanol phase. The inhibitory activities for the percentage germination of B. pekinensis seedlings and root growth of all organic phases and water phase in the testa orders were methanol phase,water phase,ethylether phase ethyl acetate phase,and petroleum ether phase. For the pericarp,the inhibitory activity orders were methanol phase,ethyl acetate phase,petroleum ether phase,ethylether phase,and water phase.