容县乌榄叶挥发油化学成分及抗氧化活性分析

GC-MS analysis and antioxidant activity of volatile oil from leaves of Canarium pimela Leenh. in Rongxian county

【目的】分析广西容县乌榄叶挥发油的化学成分及其抗氧化活性,为乌榄叶的药用开发提供参考。【方法】采用水蒸气蒸馏法提取广西容县乌榄叶中的挥发油,利用气相色谱—质谱联用法(GC-MS)分析其化学成分,通过峰面积归一法计算各成分的相对含量,并用DPPH·法对其抗氧化活性进行测定。【结果】容县乌榄叶挥发油共分离出55种成分,鉴定了53种,占挥发油总量的98.130%,其中单萜类化合物和倍半萜类化合物比例高,分别为20和18种;挥发油主要成分有雅槛兰-1(10),11-二烯(11.652%)、α-芹子烯(10.291%)、γ-依兰油烯(9.719%)、α-蒎烯(9.208%)和1,6-二甲基-4-异丙基-1,2,3,4,4a,7-六氢化萘(7.523%);乌榄叶挥发油质量浓度为1.2 g/L时,其对DPPH·的清除率为63%。【结论】容县乌榄叶挥发油化学成分种类较多,以单萜类化合物为主,且具有一定的抗氧化活性,可作为天然香料、抗氧化剂资源进一步研究。

【Objective】The chemical constituents and antioxidant activity of the volatile oil from leaves of Canarium pimela Leenh. in Rong County were studied to provide references for its medicinal development. 【Method 】The volatile oil was extracted by steam distillation, and then the composition of oil was analyzed by GC-MS. Chromatographic peak area normalization method was used to calculate the relative mass fraction of each ingredient. The DPPH· method was used to study its antioxidant activity. 【 Result 】 55 compounds were isolated and 53 compounds were identified in the leaves that composed about 98.130% of the total volatile oils, in which the ratio of monoterpenoids and sesquiterpenes was relatively higher, 20 and 18 types, respectively. The principal chemical constituents of the volatile oil were eremophila-1（10）, 11-diene（11.652%）,α-selinene（10.291%）, γ-muurolene（9.719%）, α-pinene（9.208%） and1,2,3,4,4a,7-hexahydro-1,6-dimethyl-4-（1-methylethyl）-Naphthalene（7.523%）. The scavenging ratio for the DPPH· radicals reached 63 % at the concentration of 1. 2 g / L. 【 Conclusion 】 There are many chemical constituents in the volatile oil and the main compounds are monoterpenoids. The volatile oil shows certain antioxidant activity and can be further studied as a natural spice and antioxidant.