HPLC法测定盐酸羟甲唑啉滴鼻液的含量

Determination of the Content of Oxymetazoline Hydrochloride Nasal Drops by HPLC

目的：建立高效液相色谱（HPLC）法测定盐酸羟甲唑啉滴鼻液的含量。方法：采用Diamonsil ODS C18柱（4.6 mm×250mm,5μm）,流动相为甲醇-水-三乙胺-磷酸（500∶450∶5∶1.5）,流速为1.0 mL·min^-1,检测波长279 nm,柱温25℃,进样量10μL。结果：盐酸羟甲唑啉在0.051-1.023 mg·mL^-1（r=1.000）范围内线性关系良好,平均回收率为99.8%,RSD为1.2%（n=9）。结论：本方法简便准确,重复性好,能有效控制盐酸羟甲唑啉滴鼻液的质量。

Objective： To develop High Performance Liquid Chromatography（ HPLC） method for the determination of Oxymetazoline Hydrochloride Nasal Drops. Methods： A Diamonsil C18column（ 4. 6 mm × 250 mm,5 μm） was used with a mobile phase consisting of methanol-water-triethylamine-phosphoric acid（ 500∶ 450∶ 5∶ 1. 5,v / v） at a flow rate of 1. 0 mL·min^-1. The detection wavelength was set at 279 nm,and the column temperature was 25 ℃. Results： The liner range of Oxymetazoline Hydrochloride was 0. 0512 - 1. 023 mg·mL^-1. The average recovery of Oxymetazoline Hydrochloride was 99. 8%（ RSD = 1. 2%,n = 9）. Conclusion： The method can be used to control the quality of Oxymetazoline Hydrochloride Nasal Drops which provides the advantage of quickness,simplicity and repeatability.