黑臭河道底泥细菌群落结构对人工曝气响应的DGGE分析

Responses of bacterial community structure in malodorous river sediments to aeration by DGGE

采用基于16S rDNA的PCR-DGGE(变性梯度凝胶电泳)图谱并通过条带割胶回收DNA进行序列分析,初步探讨了不同曝气条件下黑臭河道底泥中细菌群落结构多样性及其变化,同时用冗余分析(RDA)研究了环境因子与细菌群落结构之间的关系.结果表明,人工曝气对黑臭河道底泥细菌群落结构产生明显的影响,并且随不同曝气强度底泥细菌群落多样性呈现不同的变化,其中当曝气扰动雷诺数(Re)为1810,溶解氧(DO)为7.35时,细菌优势群落多样性最高;序列比对分析推测适度的曝气有利于促进碳、氮、硫循环相关细菌的生长,其中以变形菌门为主导;冗余分析显示DO和Re对细菌群落结构影响显著.

In this study,16 S rDNA based PCR amplification and denaturing gradientgel electrophoresis（PCR-DGGE）as well as sequence alignment from the excised DGGE bands were used to investigate the bacterial diversity and variation.In addition,the redundancy analysis was adopted to assess the relationship between bacterial community structure and environmental factors.The results showed that aeration had marked effects on bacterial community structure in malodorousriver sediments and different aeration intensities resulted in variable bacterial community.Amongst them the highest richness of bacterial community was observed with the aeration turbulence of Re=1 810 and DO of 7.35.It was also inferred that moderate aeration facilitated the growths of related bacteria to N-and S-cycling and theProteobacteria was dominant.It was also found that DO and Re played important roles in bacterial community from river sediments.