麻醉方法对痉挛型瘫痪大鼠动物模型构建的影响

Anesthesia Selection for Modeling Spastic Paralysis in Rats

目的探讨双侧颈总动脉间断性结扎术建立大鼠痉挛型瘫痪模型时的最适麻醉剂量及方式。方法 Wistar大鼠60只,随机分成6组,每组10只,均行双侧颈总动脉间断性结扎术。A组予10%水合氯醛5 ml/kg皮下注射,B组予10%水合氯醛4 ml/kg皮下注射,C组予10%水合氯醛4 ml/kg腹腔注射,D组予10%水合氯醛3 ml/kg皮下注射,E组予10%水合氯醛3 ml/kg腹腔注射,F组予10%水合氯醛2 ml/kg皮下注射。比较每组麻醉起效时间、维持时间、术中死亡率。结果 A组在麻醉中全部死亡,F组无深度麻醉。B组起效时间(6.5±0.7)min,维持时间(121.4±3.9)min,死亡率0;C组起效时间(5.5±1.1)min,维持时间(122.0±3.6)min,死亡率30%;D组起效时间(9.6±0.8)min,维持时间(106.7±3.7)min,死亡率0;E组起效时间(7.4±1.2)min,维持时间(105.3±3.5)min,死亡率20%。皮下注射总体死亡率0,腹腔注射总体死亡率25%。结论双侧颈总动脉间断性结扎术建立大鼠痉挛型瘫痪模型时,采用10%水合氯醛4 ml/kg皮下注射麻醉,大鼠麻醉死亡率低,起效时间短,维持时间长。

Objective To explore the optimal dose and ways of anesthesia for creating a rat model of spastic paralysis with intermittent bilateral common carotid artery ligation. Methods 60 Wistar rats were randomly divided into groups A, B, C, D, E and F. Group A was anaesthetized with 10% chloral hydrate 5 ml/kg injected subcutaneously, while group B with 4 ml/kg subcutaneously, group C with 4 ml/kg intraperitoneally, group D with 3 ml/kg subcutaneously, group E with 3 ml/kg intraperitoneally, group F with 2 ml/kg subcutaneously. The onset and duration of anesthesia, and intraoperative and postoperative mortality were compared. Results All the rats in the group A died during anesthesia, while the group F did not achieve the depth of anesthesia, and abandoned. The onset time was（6.5±0.7） min, maintained（121.4±3.9） min, mortality was 0 in the group B, and it was（5.5±1.1） min,（122.0±3.6） min, 30% in the group C;（9.6±0.8） min,（106.7±3.7） min, 0in the group D,（7.4±1.2） min,（105.3±3.5） min, 20% in the group E. The overall mortality rate was 0 in the groups accepted subcutaneous injected and 25% of intraperitoneal injection. Conclusion Anesthesia with 10% chloral hydrate 4 ml/kg subcutaneous injection is optimal of lower mortality, faster onset and longer maintaining in rats for spastic paralysis model with intermittent bilateral common carotid artery ligation.