福州地区灰霉病菌对腐霉利和嘧霉胺抗药性测定

Resistance Detection of Botrytis cinereain Fuzhou to Procymidone and Pyrimethanil

从福州福清、长乐、连江、闽清4地设施栽培的番茄、茄子、辣椒、黄瓜等蔬菜作物上采集灰霉病菌147株，采用菌丝生长速率法，分别检测灰霉病菌对腐霉利和嘧霉胺2种杀菌剂的抗药性。结果表明：福州地区灰霉病菌对腐霉利嘧霉胺都产生较高的抗性。腐霉利对灰霉病菌的 EC50范围为0.0634～10.0579μg · mL^－1，抗性频率平均值为76％，嘧霉胺对灰霉病菌的EC50范围为0.1052～102.3465μg · mL^－1，抗性频率平均值为73％， 2种杀菌剂抗性频率最高都达100％，其中对腐霉利高抗菌株70个，高抗频率达48％；对嘧霉胺高抗菌株79个，高抗频率达54％。从地域上看，福清、长乐、连江、闽清4地灰霉病菌对腐霉利和嘧霉胺抗性频率平均值分别为74％、73％、82％、73％和84％、82％、74％、51％，闽清县对2种药剂抗药性稍低，其他3个县市差异不大。从寄主作物看，番茄、茄子、辣椒、黄瓜上灰霉病菌对腐霉利和嘧霉胺抗性频率平均值分别为77％、73％、73％、78％和74％、73％、67％、76％， 4种寄主上灰霉病菌对腐霉利和嘧霉胺抗性频率差异不大。不同寄主间的发病程度与抗药性大小无显著相关。

147 Botrytis cinerea strains were isolated from tomato , eggplant , hot pepper and cucumber in the greenhouse of Fuqing ,Changle ,Lianjiang and Minqing. Resistance detection of Botrytis cinerea in Fuzhou to Procymidone and Pyrimethanil was characterized by measuring diameters of mycelial grow th. Our results showed that the resistance of Botrytis cinerea in Fuzhou to Procymidone and Pyrimethanil are high ,with EC50 values from 0.063 4-10.057 9 μg · mL ^-1 and 0.105 2 -102.346 5 μg · mL ^-1 ,and resistance frequency are 76% and 73% , separately. The highest resistant frequency for the two fungicides was both 100%. The percentage of high‐resistant isolates to Procymidone and pyrimethanil were 48% and 54% . In geographical , the resistanct frequency to Procymidone and Pyrimethanil in Fuqing ,Changle ,Lianjiang and Minqing were 74% ,73% ,82% ,73% and 84% , 82% ,74% ,51% respectively ,suggesting that the resistant frequency of Mingqing is lower than the other three places. In host plants , the resistance frequency of isolates collected from tomato , eggplant , hot pepper and cucumber to Procymidone and Pyrimethanil were 77% , 73% , 73% , 78% and 74% , 73% , 67% , 76%respectively ,suggesting that there have no resistant differences in host plants.