生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化

Optimization of Solid-state Fermentation Condition for the Biocontrol Strain of Trichoderma harzianum FJAT-9040 by Response Surface Methodology

为优化生防菌哈茨木霉FJAT-9040固体发酵条件,结合初始含水量、初始接种量及培养温度3个单因素,Box-Behnken设计和响应面分析法进行研究。以产孢量为指标,单因素试验确定初始含水量、初始接种量及培养温度分别为50%、5%、28℃。响应面分析结果表明:初始含水量和温度与产孢量有显著相关性(P<0.05),通过求解回归方程可得,当初始含水量为53%,初始接种量为7%,培养温度29℃时,产孢量达到4.04×109个·g-1,与实际值3.86×109个·g-1相近。

Optimization of solid‐state fermentation condition for the biocontrol strain of T richoderma harz ianum FJAT‐9040 using single factor analysis ,Box‐Behnken design.and response surface methodology （RSM ） . The constitute of fermentation condition initial moisture ,initial inoculation amount ,culture temperature were determined by single factor experiment ,then the optimal condition was optimized using Box‐Behnken design and RSM. The result of single factor showed that the initial moisture ,initial inoculation amount ,culture temperature were 50% , 5% ,28℃ ,respectively. The results showed that initial moisture and culture temperature were significant for the biomass of strain of T.harz ianum based on the response surface methodology analysis （ P〈 0.05 ） .T he optimum fermentation conditions were as followed ：initial moisture 53% ,initial inoculation amount 7% ,culture temperature 29℃ , the average sporulation yield reached to 4.04 ×10^9 cfu · g^-1 , which was closed to the actual 3.86 × 10^9 cfu · g^-1 .