富硒蚕蛹氨基酸对S180荷瘤小鼠抗肿瘤作用的实验研究

Experimental study on the antineoplastic effect of rich selenium of amino acid of silkworm chrysalis on S180 tumor bearing mice

目的：探讨富硒蚕蛹氨基酸对S180荷瘤小鼠的抗肿瘤作用。方法健康昆明小鼠50只，于右腋皮下接种S180肿瘤细胞，建立小鼠肿瘤模型。次日按性别、体质量完全随机分成五组：荷瘤对照组（0．9％氯化钠注射液）、阳性对照组（环磷酰胺0．03 mg／g）、富硒蚕蛹氨基酸高剂量组（0．2 mg／g）、中剂量组（0．1 mg／g）、低剂量组（0．05 mg／g）；连续给药10 d，次日摘眼球取血并测定白细胞数和血小板数，称量小鼠体内瘤重并计算抑瘤率，观察肝体比、脾体比、肺体比以及用药前后的体质量变化等指标。结果富硒蚕蛹氨基酸高剂量组、中剂量组和低剂量组与荷瘤对照组相比瘤重量明显减小，其抑瘤率分别为30．06％、25．43％和22．54％。阳性对照组白细胞和血小板较荷瘤对照组差异均有统计学意义（F＝4．27、5．33，均P＜0．01）；富硒蚕蛹氨基酸组白细胞数与荷瘤对照组相比差异无统计学意义（ F＝22．72、29．67、27．28，均P＞0．05），与阳性对照组相比差异有统计学意义（F＝4．85、5．02、5．37，均P＜0．01）；血小板较荷瘤对照组差异均有统计学意义（F＝3．57、3．93、4．14，均P＜0．01），与阳性对照组相比差异无统计学意义（F＝38．25、42．38、46．31，均P＞0．05）。与荷瘤对照组相比，高剂量组肝体比差异有统计学意义（F＝6．31，P＜0．05），肺体比、脾体比差异均无统计学意义（F＝28．25、22．34，均P＞0．05）；中剂量组肝体比差异有统计学意义（F＝4．11，P＜0．01），肺体比、脾体比差异均无统计学意义（F＝23．33、18．28，均P＞0．05）；低剂量组肝体比和肺体比差异有统计学意义（F＝3．83、4．54，均P＜0．01），脾体比差异无统计学意义（F＝19．31，P＞0．05）。富硒蚕蛹氨基酸各组的肝体比、肺体比和脾体比与阳性照组相比，差异均有统计学意义（均P＜0．05）。结论富硒蚕蛹氨基酸具有抑制S180荷瘤小鼠肿瘤生长作用，虽然抑瘤效果不如环磷酰胺，但其毒性比环磷酰胺小，具有一定的应用价值。

Objective To discuss the antineoplastic effect of rich selenium of amino acid of silkworm chrysa-lis on S180 tumor bearing mice .Methods The models of tumor bearing mice were established by hypodermic inocu-lating fifty Kunming healthy mice＇s right armpit.The mice were randomly divided into 5 groups next day according to the sex and body weight： tumor control group （0.9% sodium chloride injection ）, positive control group （ CTX 0.03mg/g）,high dosage of rich selenium of amino acid of silkworm chrysalis group （0.2mg/g）,middle dosage group （0.1mg/g）,low dosage group（0.05mg/g）.Successive medication to mice for ten days .The next day,we got blood by pick eyeball of mice ,and determine its leukocyte number and platelet number .We measured the weight of tumor and calculated the ratio of tumor restriction .The ratio of liver and body weight , spleen and body weight , lung and body weight and the change of weight after medication were observed .Results Compared to tumor control group , the weight of high dosage group middle dosage group and low dosage group were grown down obviously ,the ratio of tumor restriction were 30.06%,25.43% and 22.54%.The leukocyte number and platelet number of positive group was lower than tumor group（F=4.27,5.33,all P〈0.01）.The leukocyte number between rich selenium of amino acid of silkworm chrysalis and tumor control group had no diversity （F=22.72,29.67,27.28,all P〉0.05）,and had statisti-cal significance with positive group （F=4.85,5.02,5.37,all P〈0.01）;the platelet number was lower than tumor group（F=3.57,3.93,4.14,all P〈0.01）.and had no significance with positive group （F=38.25,42.38,46.31,all