金黄色葡萄球菌侵入成骨细胞机制初探

A primary study on the invasion mechanisms of Staphylococcus aureus into cultured human osteoblasts

目的 :探讨金黄色葡萄球菌在侵入成骨细胞过程中 ,细菌和细胞各自所起的作用。方法 :半对数生长期金黄色葡萄球菌 6 5 71为实验用菌株。成骨细胞培养自健康成人下颌第三磨牙拔除术中去除的骨块。氯霉素、利福平、新生霉素、放线菌酮和Monodansylcadaverine作为细菌或成骨细胞的抑制剂 ,研究它们在金黄色葡萄球菌侵入成骨细胞过程中的作用。结果 :氯霉素、利福平、新生霉素和Monodansylcadaverine处理组的细菌侵入能力明显低于对照组 ,放线菌酮处理组明显高于对照组。结论 :金葡菌在侵入成骨细胞过程中自身需合成蛋白质、RNA和DNA ;成骨细胞摄入细菌需借助受体介导的细胞吞饮作用 ;

Objective: To study what are needed during the invasion of Staphylococcus aureus into cultured human osteoblasts.Methods: Mid-logarithmic growing Staphylococcus aureus 6571 was prepared. Human osteoblasts were cultured from bone explants. Chloramphenicol, rifampicin, novobiocin, cycloheximide, and monodansylcadaverine were used as inhibitors of either bacteria or cells to study their roles in the invasion of Staphylococcus aureus into osteoblasts. Results: Chloramphenicol, rifampicin, novobiocin, and monodansylcadaverine decreased the invasive ability of the bacteria; while cycloheximide increased the ability.Conclusion: De novo protein as well as RNA-and DNA-synthesis by the bacteria is necessary for invasion. The inhibition of osteoblast protein synthesis increases the uptake of the bacteria.