行为学训练对局灶性脑缺血大鼠内源性神经干细胞增殖及PS1蛋白表达的影响

Effect of behavior training on expression of PS1 and endogenous neural stem cells in cerebral tissues of vascular focal cerebral ischemia rats

目的检测早老素(Presenilinl,PS1)在局灶性脑缺血大鼠海马组织神经干细胞中的表达及穿梭箱训练的干预作用,探讨影响神经干细胞增殖分化的分子调控机制。方法采用大脑中动脉栓塞(MCAO)制作局灶性脑缺血大鼠模型,用免疫组织化学方法检测海马神经干细胞Brd U、PS1的表达及穿梭箱训练的干预作用。结果随着脑缺血再灌注3 d缺血海马神经元Brd U阳性细胞明显增多,7 d达高峰。PS1反应产物脑缺血再灌注7 d较正常组增多,随缺血再灌注时间的进一步延长逐渐减少,14 d后持续低水平表达。穿梭箱训练后Brd U、PS1蛋白的表达明显增加。结论穿梭箱训练促进神经干细胞的增殖及缺血脑组织PS1的表达,提示穿梭箱训练促进神经干细胞增殖作用可能由PS1信号介导。

Objective To investigate the expression of PS1 expression and hippocampal neurogenesis in a rat model of cerebral ischemia / reperfusion,and to explore the related molecular and behavior training mechanism. Methods Wistar rats were randomized into training groups,ischemia and reperfusion groups and control group. Behavior training was performed at day 1 after the ischemia and reperfusion. The model of middle cerebral arteries occlusion（ MCAO） were performed with intraluminal filament blockade. The expression of Brd U and PS1 and in the hippocampus was detected with immunohistochemical methods. Results After 3 days,Brd U positive cells in the hippocampus were obviously increased. On day 7,Brd U positive cells were more than those at any time. PS1 reaction products were increased gradually with the time of cerebral ischemia / reperfusion,reached the peak on 7 day,and then gradually decreased. After training,the expression of hippocampal PS1 had apparently increased in each group. Conclusion Behavior training promoted the expression of PS1 in the hippocampus tissues. These findings indicate that training promotion of neural stem cell proliferation may be mediated by PS1 signaling pathway.