高氯酸盐高效降解菌的筛选及降解特性

STUDY ON THE SCREENING AND DEGRADATION CHARACTERISTICS OF HIGHLY EFFICIENT PERCHLORATE DEGRADING CONSORTIUM

从长春市某河道的底泥中筛选出能够降解高氯酸盐的一组菌群。经PCR扩增及DGGE分离后,对V3区进行测序鉴定,结果显示该菌群主要由Pantoeasp、Klebsiellasp、Enterobactersp、Citrobactersp、Erwiniasp、Sulfurospirillumsp、Epsilon proteobacterium等细菌构成。驯化后,该菌群能够高效降解高氯酸根,在最佳接菌量为2%、pH为7.0,高氯酸根的初始质量浓度为100-1 000 mg/L时,其对高氯酸根的降解半衰期为5-30 h,降解动力学均为零级反应。利用离子色谱测试分析该菌群降解高氯酸盐的代谢途径,得出最终降解产物为氯离子,无其它中间产物的存留。

A group of bacteria was screened from the sediment of a river in Changchun,Jiling Province,China,which could degrade perchlorate.After PCR amplification and DGGE separation,the V3 region was sequenced for DNA identification.The results of sequence demonstration showed that the bacteria flora was mainly composed by the following bacteria：Pantoeasp,Klebsiellasp,Enterobactersp,Citrobactersp,Erwiniasp,Sulfurospirillumsp,Epsilon proteobacterium,etc.After domestication,perchlorate could be efficiently degraded by this bacteria flora.When the bacteria inoculation amount and pH value were 2% and 7.0,respectively,the initial concentration of perchlorate was in the range of 100-1 000 mg/L with its half-life was 5-30 h,and the zero order reaction was found to be the most suitable to describe degradation kinetics.It was found that chloride ion was the final product without any other intermediate product through the ion chromatography analysis of metabolic pathways derived from degradation of perchlorate.