哺乳期磺胺间甲氧嘧啶暴露对仔鼠行为影响

Effects of maternal sulfamonomethoxine exposure during lactating on behaviors of offspring mice

目的探讨磺胺间甲氧嘧啶(SMM)哺乳期暴露对青春期小鼠探索和焦虑行为影响。方法小鼠哺乳期以0(对照组)、10、50、200 mg/kg灌胃染毒,连续21 d,断乳后其仔鼠雌、雄分笼正常喂养至49 d,次日进行旷场、黑白巷和高架十字迷宫实验。结果与对照组雄鼠比较,低、中剂量SMM组雄鼠旷场实验中央区域时间[分别为(40.6±10.0)、(41.0±11.9)s]增加,差异有统计学意义(P<0.05),中剂量组雄鼠站立次数[(56.5±8.8)次]明显增加,差异有统计学意义(P<0.01);与对照组雌鼠比较,低剂量SMM组雌鼠旷场实验中央区域时间[(37.3±9.0)s]增加,差异有统计学意义(P<0.05),中剂量SMM组雌鼠的跨越格子数[(159.1±26.0)个]增加,差异有统计学意义(P<0.05);高剂量SMM组雌鼠黑白巷实验黑端时间[(57.8±8.2)s]增加,差异有统计学意义(P<0.01)。结论哺乳期SMM暴露可干扰仔鼠的探索和焦虑等行为。

Objective To explore the effects of maternal sulfamonomethoxine（SM M） exposure during lactating on exploratory and anxiety-related behaviors of offspring mice in puberty. Methods The lactational mice were orally administered with SM M（0,10,50,200 mg/kg） for 21 days. Then the offspring mice were separated by gender and raised normally until postnatal day 49,and their exploratory and anxiety-related behaviors were detected with open-field,blackw hite alley and elevated shelf plus maze test the next day. Results Compared with the control,the central region time of the male offspring mice with 10 and 50 mg/kg maternal SMM exposure increased significantly（40. 6 ± 10. 0 and 41. 0 ± 11. 9seconds,P〈0. 05）;the standing times in the open-field test for the male offspring mice with 50 mg/kg maternal SM Mexposure increased significantly（56. 5 ± 8. 8 seconds,P〈 0. 01）. For the female offspring mice compared to those of the control,the central region time significantly increased（37. 3 ± 9. 0 seconds） in 10 mg/kg maternal SM Mexposure group（P〈0. 05）;the total number of squares crossed in the open-field test significantly increased（159. 1 ± 26. 0） in 50 mg /kg SM Mgroup（P 〈0. 01）; and the total time spent in the black half test was also significantly increased（57. 8 ± 8. 2seconds） in 10 mg/kg SM Mgroup（P〈0. 05）. Conclusion The results indicate that maternal SM Mexposure during lactation affects exploratory and dysphoric behaviors of the offsprings mice in puberty.