超声波诱导钙黄绿素脂质体缓释特性评价

Ultrasound Induced Release Property Evaluation of Calcein Encapsulated Liposome

在脂质体药物缓释研究中,超声波因其无创、易控制等优点而备受关注。采用去离子水制备聚乙二醇修饰的钙黄绿素脂质体,并用负染色法对超声波作用前后的脂质体外观形态进行了透射电镜观测。同时采用阻抗分析法测定脂质体溶液的阻抗变化,以评价超声波作用后脂质体内包药物的释放特性。结果表明:超声波可促进多层脂质体膜的融合形成单层膜结构,最终将脂质体破坏成微小胶束,并且可通过调整超声波的作用时间和功率,控制药物的释放速率。

In the study of liposome controlled release,ultrasound has been attracted great interests because of its merits of non-invasive and easily to be controlled. Calcein trapped PEG-liposome was prepared with de-ionized water and morphology of the liposomes before and after ultrasonication were studied by transmission electron microscopy（ TEM） with negative staining method. An impedance evaluation method was employed to monitor the impedance change of liposomes thus to evaluate the liposome＇s release property. Results show that ultrasound could accelerate membrane fuse of liposome＇s multilayer and would destroy liposomes into micelles finally. By adjusting ultrasound power and ultrasonication time,the release rate could be well controlled.