OCT4、SOX2和NANOG在胆囊癌中的表达及临床意义

Expression and Significance of OCT4,SOX2 and NANOG in Gallbladder Carcinoma

目的检测OCT4、SOX2和NANOG在胆囊癌组织和非癌胆囊黏膜中的表达,探讨其与胆囊癌临床病理之间的关系。方法随机选取河南科技大学第三附属医院病理科2011年10月至2014年2月经外科手术切除且病理确诊的胆囊癌及良性胆囊病变组织标本各30例,应用免疫组化方法检测OCT4、SOX2和NANOG在胆囊癌及良性胆囊病变组织中的表达,分析其临床病理意义。结果 OCT、SOX2和NANOG蛋白在胆囊癌组织中阳性表达均明显高于其在非癌胆囊病变组织中的阳性表达,分别为(83.33%vs 23.33%;76.67%vs 20%;76.67%vs20%),以上各组组间差异均有统计学意义(P<0.05)。OCT4、SOX2和NANOG三者之间表达呈正相关关系(P<0.05)。结论 OCT4、SOX2和NANOG蛋白在胆囊癌组织中的异常表达说明其与胆囊癌的发生、发展有相关性,且OCT4、SOX2、NANOG表达与胆囊癌患者的临床分期及肿瘤的分化程度有关,而与患者的年龄、性别、肿瘤大小等因素无关,联合检测有助于明确肿瘤的生物学特性。

Objective This paper aims to explore the correlation of OCT4,SOX2 and NANOG proteins and its relationship with clinical pathological features by detecting both the expressions of OCT4,SOX2 and NANOG proteins in gallbladder carcinoma. Methods From the Third Affiliated Hospital of Henan University of Science and Technology,we selected 30 surgical specimens of resected and pathologic diagnosed gallbladder carcinoma as an experimental group and selected 30 cases of benign lesions of gallbladder in the same period as a control group（ from Oct. 2011 to Feb. 2014）. Immunohistochemistry was used to detect the expressions of OCT4,SOX2 and NANOG proteins separately in gallbladder carcinoma and normal tissue,to explore the relationship between OCT4,SOX2 and NANOG proteins and gallbladder carcinoma,to analyze the relationship between OCT4,SOX2,NANOG proteins and clinical pathological features of gallbladder carcinoma. Results The positive expressions of OCT4,SOX2 and NANOG proteins in gallbladder carcinoma are significantly higher than gallbladder benign lesions,the positive rate were that 83. 33% vs23. 33% and 76. 67% vs 20% and 76. 67% vs 20%. There were statistically significant difference between the groups（ P〈0. 05）. Expression was positively correlated relationship between the OCT4,SOX2 and NANOG proteins（ P〈0. 05）. Conclusion It is clear that positive expression of OCT4,SOX2 and NANOG proteins in gallbladder carcinoma is much higher than that in the control group of gallbladder benign lesions tissues,which shows that OCT4,SOX2 and NANOG proteins may play a certain role in the occurrence and development of gallbladder carcinoma.